We narrowed to 159,461 results for: addgene

1. pHAGE EF1α dCas9-NED

   Plasmid

   #109369

   Purpose

   Lentiviral vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIGII-TCR-E

   Plasmid

   #194391

   Purpose

   Retroviral expression of candidate orphan TCR

   Depositor

   Michael Carroll

   Article

   Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

   Insert

   TCR-E (Tcra, Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-GRAB_Ado1.0mut

   Plasmid

   #153287

   Purpose

   Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN837

   Plasmid

   #184825

   Purpose

   Expresses human KIF5A in mammalian cells

   Depositor

   Kyoko Chiba

   Article

   Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

   Insert

   KIF5A (KIF5A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Flex-rev-oChIEF-tdTomato

   Plasmid

   #30541

   Purpose

   Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

   Depositor

   Rory McQuiston

   Article

   Bell et al Neuropharmacology. 2011 Aug 25.

   Insert

   oChIEF-tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB1-A-TelR

   Plasmid

   #204979

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVCT-rtTR-KRAB-2SM2

   Plasmid

   #11779

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

    Plasmid

    #136262

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001 . 1():4.

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGDP2 MCR-1

    Plasmid

    #118404

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    MCR-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2 8xSTAR-sTomato-NLS. PGK-puro

    Plasmid

    #136261

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti GFAABC1D-dCas9-KRAB-MeCP2

    Plasmid

    #194701

    Purpose

    Expresses dCas9-KRAB-MeCP2 in Astrocytes

    Depositor

    Timothy Jarome

    Article

    Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

    Insert

    GFAABC1D promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    None

    Promoter

    GFAABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 Rheb1 shRNA #2

    Plasmid

    #26626

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pAV-CAG-Sun1-GFP-WPRE-pA

    Plasmid

    #220752

    Purpose

    Expression of Sun1GFP for nuclei isolation

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    Sun1-GFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer20-EGFP-TEADiv2

    Plasmid

    #228876

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP-TEAD inhibitor (TEADiv2)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1a-NucGFP

    Plasmid

    #184706

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

    Plasmid

    #154817

    Purpose

    Inducible expression of AsiSI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AsiSI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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