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Plasmid#146527PurposeInsect Expression of DmNot4_187-1051DepositorInsertDmNot4_187-1051 (CG31716 Fly)
UseTagsExpressionInsectMutation7 silent mutations and 5 non silent mutations G20…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-EGFP-V5His6_F
Plasmid#146260PurposeInsect Expression of EGFPDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP-A_G
Plasmid#146346PurposeInsect Expression of Dm4EHP-ADepositorInsertDm4EHP-A (4EHP Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence from …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPABPC1-V5His6_G
Plasmid#146352PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and seven silent mu…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRox8-D_G
Plasmid#146354PurposeInsect Expression of DmRox8DepositorInsertDmRox8 (Rox8 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmXRN1_1-573_G
Plasmid#146355PurposeInsect Expression of DmXRN1_1-573DepositorInsertDmXRN1_1-573 (pcm Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmXRN1_574-1613_G
Plasmid#146356PurposeInsect Expression of DmXRN1_574-1613DepositorInsertDmXRN1_574-1613 (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmUPF1-V5His6_E
Plasmid#146165PurposeInsect Expression of DmUPF1DepositorInsertDmUPF1 (Upf1 Fly)
UseTagsExpressionInsectMutationtwo non silent mutations E531G and G995E compared…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-cb5-pEGFP-C1
Plasmid#177431PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationBik MBR removed (after position 136) and replaced…PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-delMBR-pEGFP-C1
Plasmid#177432PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationBik MBR removed (after position 136)PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-cb5-pEGFP-C1
Plasmid#177407PurposeTo express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.DepositorInsertCb5 tail anchor (Cyb5r4 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-BclXL-ActA-pEGFP-C1
Plasmid#177410PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-XL-ActA, BclX-acta
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-ActA-pEGFP-C1
Plasmid#177412PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-XL-ActA, BclX-acta
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-Cb5-pEGFP-C1
Plasmid#177414PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-XL-Cb5, BclX-cb5
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-BclXL-Cb5-pEGFP-C1
Plasmid#177415PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-XL-Cb5, BclX-cb5
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-ActA-pEGFP-C1
Plasmid#177418PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bcl2-ActA-pEGFP-C1
Plasmid#177419PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-cb5-pEGFP-C1
Plasmid#177421PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bcl2-cb5-pEGFP-C1
Plasmid#177422PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-L61G-pEGFP-C1
Plasmid#177426PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationLeucine 61 replaced with glycinePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-GG153AA-pEGFP-C1
Plasmid#177427PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationGlycines 153 and 154 mutated to alaninePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-G154STOP-pEGFP-C1
Plasmid#177428PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationGlycine 154 replaced with STOP codonPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
UseTags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-panTRK-CR1-EGFP
Plasmid#187194PurposeTo express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3DepositorInsertCR1 pan-TRK receptor
UseRetroviralTagsEGFPExpressionMammalianMutationPromotermo-MLV or MMLVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-tdTomato-2A-mSyp::EGFP
Plasmid#191213PurposeExpresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.DepositorInserttdTomato-T2A-mSyp::EGFP (Syp Mouse)
UsePcagenTagsmSyp::EGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKC530 pBABE EGFP-ZNF335
Plasmid#192698PurposeRetroviral transgene expression of human ZNF335DepositorInsertZNF335 (ZNF335 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-HsHelz_1475-1942_AH
Plasmid#148899PurposeMammalian Expression of HsHelz_1475-1942DepositorInsertHsHelz_1475-1942 (HELZ Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1_1-671_AI
Plasmid#148943PurposeMammalian Expression of HsGIGYF1_1-671DepositorInsertHsGIGYF1_1-671 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1-F306AF312A_AI
Plasmid#148945PurposeMammalian Expression of HsGIGYF1-F306AF312ADepositorInsertHsGIGYF1-F306AF312A (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1-W294A_AI
Plasmid#148946PurposeMammalian Expression of HsGIGYF1-W294ADepositorInsertHsGIGYF1-W294A (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1-W294AF306AF312A_AI
Plasmid#148947PurposeMammalian Expression of HsGIGYF1-W294AF306AF312ADepositorInsertHsGIGYF1-W294AF306AF312A (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718_AI
Plasmid#148948PurposeMammalian Expression of HsGIGYF2_1-718DepositorInsertHsGIGYF2_1-718 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_280-310_AI
Plasmid#148951PurposeMammalian Expression of HsGIGYF2_280-310DepositorInsertHsGIGYF2_280-310 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI
Plasmid#148952PurposeMammalian Expression of HsGIGYF2-F300AF306ADepositorInsertHsGIGYF2-F300AF306A (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-W288A_AI
Plasmid#148953PurposeMammalian Expression of HsGIGYF2-W288ADepositorInsertHsGIGYF2-W288A (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-W288AF300AF306A_AI
Plasmid#148954PurposeMammalian Expression of HsGIGYF2-W288AF300AF306ADepositorInsertHsGIGYF2-W288AF300AF306A (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-C313AL317A_AI
Plasmid#148955PurposeMammalian Expression of HsRck-C313AL317ADepositorInsertHsRck-C313AL317A (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-CLLKtoAAAA_AI
Plasmid#148956PurposeMammalian Expression of HsRck-CLLKtoAAAADepositorInsertHsRck-CLLKtoAAAA (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-L338AK342A_AI
Plasmid#148957PurposeMammalian Expression of HsRck-L338AK342ADepositorInsertHsRck-L338AK342A (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO1-P588GA618FK658AL692Y_AH
Plasmid#148860PurposeMammalian Expression of HsAGO1-P588GA618FK658AL692YDepositorInsertHsAGO1-P588GA618FK658AL692Y (AGO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only