We narrowed to 3,616 results for: mak

1. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

   Plasmid

   #138482

   Purpose

   Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Ribozyme-flanked gRNA and AmCyan
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet-1_6xHis-TEV-mCh-NDP52

   Plasmid

   #187829

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   6xHisTag, TEV, and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB_GST-TEV-SRC (Y530F)

   Plasmid

   #223743

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   Src Kinase (SRC Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Insect

   Mutation

   Y530F constitutively active mutant

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

   Plasmid

   #176085

   Purpose

   EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT2 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

   Plasmid

   #176074

   Purpose

   EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIG-IRF4

   Plasmid

   #58987

   Purpose

   MSCV based expression vector - co-expresses GFP and murine IRF4

   Depositor

   David Baltimore

   Article

   So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

   Insert

   Mouse IRF4 (Irf4 Mouse)
   

   Use

   Retroviral

   Tags

   Kozak sequence added

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas-MmdCas12m-CBE1

   Plasmid

   #192284

   Purpose

   MmdCas12m-CBE1 expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m-CDA-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-FuG-E

   Plasmid

   #67509

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

   Insert

   Fusion Glycoprotein type E
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55644

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG_GST-TEV-EGFP-ATG13

    Plasmid

    #223797

    Purpose

    Expression of recombinant protein for purification

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST-TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-hSyn-ASAP4b-Kv-WPRE

    Plasmid

    #201030

    Purpose

    Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4b-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mIFP12-Fibrillarin-7

    Plasmid

    #56252

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55649

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRXIP-GFP-CERT(PHD)(W33A)

    Plasmid

    #221761

    Purpose

    Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–116, mutated 33W to 33A

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55646

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-Con/Foff 2.0-EYFP

    Plasmid

    #137162

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

    Plasmid

    #223213

    Purpose

    Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    TGFb1 active - membrane (TGFB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge