Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,475 results for: pcdna

Showing: 3691 - 3720 of 12475 results

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)

Plasmid

#231380

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97E, K98E

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)

Plasmid

#231383

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R710A, H712A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Synthetic, Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S724A

Plasmid

#224735

Purpose

For expression of HA-tagged GTSE1 protein with S724A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S734A

Plasmid

#224736

Purpose

For expression of HA-tagged GTSE1 protein with S734A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 734 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S171A

Plasmid

#224723

Purpose

For expression of HA-tagged GTSE1 protein with S171A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 171 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S187A

Plasmid

#224724

Purpose

For expression of HA-tagged GTSE1 protein with S187A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 187 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S262A

Plasmid

#224725

Purpose

For expression of HA-tagged GTSE1 protein with S262A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 262 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S345A

Plasmid

#224726

Purpose

For expression of HA-tagged GTSE1 protein with S345A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 345 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S417A

Plasmid

#224727

Purpose

For expression of HA-tagged GTSE1 protein with S417A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 417 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S480A

Plasmid

#224728

Purpose

For expression of HA-tagged GTSE1 protein with S480A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 480 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S555A

Plasmid

#224729

Purpose

For expression of HA-tagged GTSE1 protein with S555A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 555 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S594A

Plasmid

#224730

Purpose

For expression of HA-tagged GTSE1 protein with S594A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 594 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S599A

Plasmid

#224731

Purpose

For expression of HA-tagged GTSE1 protein with S599A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 599 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S611A

Plasmid

#224732

Purpose

For expression of HA-tagged GTSE1 protein with S611A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 611 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S707A

Plasmid

#224733

Purpose

For expression of HA-tagged GTSE1 protein with S707A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 707 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S718A

Plasmid

#224734

Purpose

For expression of HA-tagged GTSE1 protein with S718A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 718 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S84A

Plasmid

#224720

Purpose

For expression of HA-tagged GTSE1 protein with S84A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 84 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S91A

Plasmid

#224721

Purpose

For expression of HA-tagged GTSE1 protein with S91A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 91 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S157A

Plasmid

#224722

Purpose

For expression of HA-tagged GTSE1 protein with S157A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 157 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3puro-EGFP-GAPDH-CC

Plasmid

#225476

Purpose

Express mEGFP-GAPDH-CC fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with CC2 domain of ROCK2 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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