We narrowed to 6,962 results for: tet on

1. NLS-dCas9-HA-NLS-GFP4-VPR

   Plasmid

   #183924

   Purpose

   VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP4-VPR
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEos2-CD151-7

   Plasmid

   #57358

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   CD151 (CD151 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pPyCAG-NE-EGFP-IRES-Pac

   Plasmid

   #183617

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

   Insert

   EGFP
   

   Use

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRJPaph-HcRed

   Plasmid

   #67035

   Purpose

   Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   HcRed
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFE-vcDAB

   Plasmid

   #133004

   Purpose

   pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   vcdabB
   vcdabA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p5B3-T

   Plasmid

   #92021

   Purpose

   Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lysA with N-terminal TEV protease cleavage site
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Expression

   Mutation

   S219V

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. gRD21A-mRFP

   Plasmid

   #181959

   Purpose

   RD21A protein tagged with mRFP, under control of native promoter

   Depositor

   George Haughn

   Article

   Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

   Insert

   RD21A genomic sequence including 2kb upstream of the ATG
   

   Use

   Tags

   mRFP

   Expression

   Plant

   Mutation

   Promoter

   RD21A native

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK2067

   Plasmid

   #171845

   Purpose

   C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

   Depositor

   Judith Kimble

   Article

   Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

   Insert

   his-58, eGFP, mCherry
   

   Use

   Tags

   N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

   Expression

   Worm

   Mutation

   boxB wild type and hairpin mutant

   Promoter

   mex-5 promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ133D2.0

   Plasmid

   #99894

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU3

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ131D2.0

    Plasmid

    #100044

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSW002-Pc-TorT(sp)

    Plasmid

    #111255

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT signal peptide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJZC40

    Plasmid

    #62334

    Purpose

    sgRNA + 2x PP7 with mCherry for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x PP7
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175017

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    OK-HEFwtED
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTPTP alpha (S204A)

    Plasmid

    #17694

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S204A (PTPRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S204A

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. p5A1-Ti

    Plasmid

    #92018

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V, C151A

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZS1[LacI-L]

    Plasmid

    #60745

    Purpose

    Contains PI driving expression dimeric LacI, the Lactose inducible repressor.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    wt LacI without the tetramerization domain

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p5A7-T

    Plasmid

    #92020

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p5A3-T

    Plasmid

    #92019

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHL600

    Plasmid

    #37563

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    CsrB (csrB E. coli)
    CsrA (csrA E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCnRed

    Plasmid

    #232908

    Purpose

    Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    gam (gam Synthetic, phage Lambda)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pbad-rha

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLD-puro-Cc-IFIT5_K206R-VA

    Plasmid

    #191224

    Purpose

    Lentiviral expression of human IFIT5_K206R in mammalian cells

    Depositor

    Mohan Babu

    Insert

    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag

    Expression

    Mammalian

    Mutation

    changed Lysine 206 to Arginine (K206R)

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-NFHΔ36-72

    Plasmid

    #232060

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 36-72
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-NFHΔ462-638

    Plasmid

    #232061

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 462-638
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-NFLtail

    Plasmid

    #232055

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFL tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Light tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-NFMtail

    Plasmid

    #232056

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-NFMshuf1

    Plasmid

    #232058

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain, charge shuffle 1
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-NFHtail

    Plasmid

    #232057

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28-NFMshuf2

    Plasmid

    #232059

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain, charge shuffle 2
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHM148

    Plasmid

    #225532

    Purpose

    Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    rtTA
    Pcdh19 (Pcdh19 Mouse)
    mCherry
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDisplay-PGD2-1.1-IRES-mcherry

    Plasmid

    #214763

    Purpose

    Express GRAB-PGD2-1.1 sensor and membrane tethered mCherry under CMV promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p2H12ref

    Plasmid

    #221188

    Purpose

    cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen) and Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMamCol1

    Plasmid

    #215184

    Purpose

    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

    Depositor

    Krishna Kishore Inampudi

    Insert

    tsPurple
    

    Use

    AAV, CRISPR, and Synthetic Biology ; Recombinant …

    Tags

    SNAC-StrepII tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetON 3G Bidirectional for Mammalian and Trc with…

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDisplay-PGD2-1.0-IRES-mcherry

    Plasmid

    #214762

    Purpose

    Express GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.0 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. JAB3278

    Plasmid

    #190402

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. JAB2601

    Plasmid

    #190388

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. JAB2600

    Plasmid

    #190387

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. JAB2599

    Plasmid

    #190386

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPBO.ACT002

    Plasmid

    #182712

    Purpose

    aTc inducible dCasRx-IF1

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 1

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNB207

    Plasmid

    #169118

    Purpose

    TESEC expression vector for DapB from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    dapB (dapB Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

    Plasmid

    #170488

    Purpose

    SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. tcTRP24sub6

    Plasmid

    #175795

    Purpose

    de novo thick circular tandem repeat protein tetramer formed from 6-repeat monomers

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP24sub6
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pKB238

    Plasmid

    #170033

    Purpose

    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Neuropeptide Y (NPY Human, Synthetic, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pKB235

    Plasmid

    #170024

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 (CCL20 Human, Synthetic, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. EZH2-Promoter-gRNA

    Plasmid

    #131329

    Purpose

    gRNA to generate endogenously tagged -tetR-EZH2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EZH2-Promoter-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTW927

    Plasmid

    #115961

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 ORF TetR-2A-mKate-PEST GG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pFE-dabAB2 C539A

    Plasmid

    #133008

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C539A

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pFE-dabAB2 H524A

    Plasmid

    #133007

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H524A

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pFE-dabAB2 D353A

    Plasmid

    #133006

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSTAP-seq_fly-4xUAS

    Plasmid

    #125149

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pJB557

    Plasmid

    #115967

    Purpose

    SGP insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 -98/16 SGP 2xTetApt-STOP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge