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pnEA-NpM-Hs4EBP3_36-69-Y40AL45A-GB1_AA

Plasmid

#148280

Purpose

Bacterial Expression of Hs4EBP3_36-69-Y40AL45A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-Y40AL45A (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-YLLVtoAAA-GB1_AA

Plasmid

#148281

Purpose

Bacterial Expression of Hs4EBP3_36-69-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-YLLVtoAAAA (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB

Plasmid

#148372

Purpose

Insect Expression of Dm4E-T-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-F41DW42D (4E-T Fly)

Expression

Insect

Mutation

5 non silent mutation L127S, E493G, H628R, N659S,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_AB

Plasmid

#148373

Purpose

Insect Expression of Dm4E-T-YLFWtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-YLFWtoAADD (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations H611R and N659S and 8 si…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_32-83-GB1_AA

Plasmid

#148267

Purpose

Bacterial Expression of DmThor_32-83

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_32-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-hCD271-PGK-Neo

Plasmid

#186171

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

hCD271

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-EGFP-PGK-Puro

Plasmid

#186167

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UIM-mut

Plasmid

#187147

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

A1376G/S1382A

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_F3194S

Plasmid

#187140

Purpose

mammalian cell expression of FLAG-HUWE1 F3194S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

F3194S

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_H669Q

Plasmid

#187138

Purpose

mammalian cell expression of 3xFLAG-HUWE1 H669Q

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

H669Q

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM-mut

Plasmid

#187151

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBM-mut

Plasmid

#187149

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

L2976A/P2977A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA-mut

Plasmid

#187145

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

M1328A/F1330A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_BH3-4E

Plasmid

#187143

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta169-189

Plasmid

#187134

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta169-189

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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