We narrowed to 2,923 results for: COB;

1. pCMV-Flag YAP S381A

   Plasmid

   #27377

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   S381A

   Promoter

   Available Since

   March 4, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pJV53-Cas12a

   Plasmid

   #158706

   Purpose

   CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR1068

   Plasmid

   #111092

   Purpose

   BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   BlpI site disrupted

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. myc-hIFITM3-delta1-21

   Plasmid

   #104365

   Purpose

   Expresses human IFITM3-delta1-21 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095.

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21

   Promoter

   CMV

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-myc-hIFITM3

   Plasmid

   #58461

   Purpose

   Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   Sept. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCR1056

   Plasmid

   #71824

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDE43-MCK

   Plasmid

   #49523

   Purpose

   Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Flag-YAP-S94/F95A

   Plasmid

   #33057

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S94A, F95A

   Promoter

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-HA-mIFITM1

    Plasmid

    #58415

    Purpose

    Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

    Insert

    IFITM1 (Ifitm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-Flag-YAP-S384A

    Plasmid

    #33070

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S384A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-Flag-YAP-del 50-59

    Plasmid

    #33065

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 50-59

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. Human SOX17 sgRNA

    Plasmid

    #59725

    Purpose

    Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hSox17 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Human BLIMP1 sgRNA

    Plasmid

    #59724

    Purpose

    Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hBLIMP1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCMV-Flag-YAP-S387A

    Plasmid

    #33071

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S387A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-Flag-YAP-D383A

    Plasmid

    #33074

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D383A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-Flag-YAP-S387D

    Plasmid

    #33073

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S387D

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pMV306-ribo-Sec-APEX2

    Plasmid

    #111701

    Purpose

    Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    V5 and mpt63 signal sequence

    Expression

    Bacterial

    Mutation

    Promoter

    hsp60-ribo

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR1323

    Plasmid

    #111125

    Purpose

    Expresses non-targeting_01224 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    Non-targeting gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge