We narrowed to 3,254 results for: actin

1. iSM_PPL2-IP

   Plasmid

   #235989

   Purpose

   Expresses iSM_PPL2 in mammalian cells

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

   Insert

   iSM_PPL2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   replacement of 5 amino acids of Smac-Cas9 PL2 wit…

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LentiCRISPR-B_sgRNA_D11 (pAVA3773)

   Plasmid

   #239310

   Purpose

   Lentiviral vector expressing Cas9-P2A-Blasticidin and a non-targeting sgRNA as control(-)

   Depositor

   Andrei Alexandrov

   Article

   Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

   Insert

   U6-driven non-targeting sgRNA Control(-)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMDC32B-B/c

   Plasmid

   #227963

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TAK4

   Plasmid

   #227094

   Purpose

   Luciferase reporter vector with MMTV 5' Element cloned downstream of luciferase gene in pHMRΔeluc

   Depositor

   Farah Mustafa

   Article

   Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

   Insert

   MMTV insert spanning from R region to 400 bp of Gag
   

   Use

   Luciferase

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF-BOS-LGP2-Myc

   Plasmid

   #215874

   Purpose

   LGP2-Myc expression vector for overexpression

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

   Insert

   LGP2 (DHX58 Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGAD424-Rabex-5

   Plasmid

   #197043

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   Rabex-5 (RABGEF1 Bovine)
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENmRFPCNAL2 pc1054

   Plasmid

   #167567

   Purpose

   The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

   Depositor

   Cristina Cardoso

   Article

   Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPLN-hFluc-DM-HA-GFP11-KDEL

   Plasmid

   #177723

   Purpose

   DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFlucDM
   

   Tags

   GFP11, HA, KDEL, and Prolactin Signal Sequence

   Expression

   Bacterial and Mammalian

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #175801

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFendoNA2S

    Plasmid

    #174730

    Purpose

    Expresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    endoNA2-S706A
    

    Expression

    Bacterial

    Mutation

    Changed Serine 706 to Alanine

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDK616

    Plasmid

    #134202

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    K223A K226A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIW040

    Plasmid

    #134127

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    aa 132-255 R236A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIW039

    Plasmid

    #134126

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    aa 132-255 and K203A K206A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIW028

    Plasmid

    #134125

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    aa 132-255 and K183A K184A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIW070

    Plasmid

    #134138

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    residues 1-131, CRISPR resistant

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3 FLAG_CMV14/OCTN2 (delta14_22)

    Plasmid

    #112800

    Purpose

    Expression of OCTN2 mutant

    Depositor

    Katarzyna Nalęcz

    Article

    Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

    Insert

    OCTN2/SLC22A5 (Slc22a5 Rat)
    

    Tags

    3 x Flag

    Expression

    Mammalian

    Mutation

    amino acids 14_22 deleted

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tetO-MITF

    Plasmid

    #170695

    Purpose

    doxycycline-inducible overexpression of MITF

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MITF (MITF Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge