We narrowed to 6,934 results for: crispr cas9 plasmids

1. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. NDUFAB1 C3.3 gRNA

   Plasmid

   #90791

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C3.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NDUFAB1 C4.3 gRNA

   Plasmid

   #90793

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSc1-DD

   Plasmid

   #80439

   Purpose

   Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Tags

   E. coli dihydrofolate reductase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2

   Plasmid

   #87360

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-2A-eGFP
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Tags

   Cas9-T2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-multi-Guide

   Plasmid

   #85401

   Purpose

   Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPATZ1.1.0-gDNA

   Plasmid

   #132476

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   PATZ1 (PATZ1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF449-R.1.0-gDNA

   Plasmid

   #132469

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF449 (ZNF449 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZNF302.1.0-gDNA

   Plasmid

   #132467

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF302 (ZNF302 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZNF253.1.0-gDNA

    Plasmid

    #132461

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF253 (ZNF253 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZFPM2.1.0-gDNA

    Plasmid

    #132459

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZFPM2 (ZFPM2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZNF3.1.0-gDNA

    Plasmid

    #132440

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF3 (ZNF3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSHZ1.1.0-gDNA

    Plasmid

    #132434

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TSHZ1 (TSHZ1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_HHEX_1

    Plasmid

    #72354

    Purpose

    Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    HHEX (HHEX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_HHEX_2

    Plasmid

    #72355

    Purpose

    Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    HHEX (HHEX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid

    #99690

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid

    #99694

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUDP046

    Plasmid

    #107062

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. HCP5

    Plasmid

    #166107

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GE-0010-gRNA2-MpCKB

    Plasmid

    #238528

    Purpose

    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta

    Depositor

    Michael Hothorn

    Insert

    MpCKB
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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