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pLenti-TRE-FH-TET3 short

Plasmid

#166916

Purpose

Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

TET3 short isoform

Use

Lentiviral

Tags

HA Tag

Expression

Mammalian

Mutation

Changed nucleotide 18 (G) to (A) to delete FseI r…

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGenLenti V5-BNIP3

Plasmid

#223732

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 (BNIP3 Human)

Use

Lentiviral

Tags

V5

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)

Plasmid

#223770

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Mutation

R108E/R125E

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTarget: Ptaq-RFP_PlacIq-GFP

Plasmid

#192280

Purpose

RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Inserts

RFP
GFP

Expression

Bacterial

Promoter

PlacIq and Ptaq

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

Plasmid

#81022

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

5’LTR

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pCX0393

Plasmid

#205984

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_12E

Plasmid

#98654

Purpose

Expresses MBP-tagged phosphomimetic FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP12-CD81-10

Plasmid

#56248

Purpose

Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CD81 (CD81 Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMRXIP-GFP-FAPP(PHD)

Plasmid

#221748

Purpose

Stable expression of GFP-FAPP(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

FAPP (PLEKHA3 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–101

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-OSBP(PHD)

Plasmid

#221745

Purpose

Stable expression of GFP-OSBP(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

OSBP (OSBP Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 87–185

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-NpHR3.3-EYFP

Plasmid

#137151

Purpose

Viral expression of NpHR3.3-EYFP

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

NpHR3.3-EYFP

Use

AAV

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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tdKatushka2-Fibrillarin-7

Plasmid

#56041

Purpose

Localization: Nucleoli, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

Fibrillarin (FBL Human)

Tags

tdKatushka2

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLKO-Tet-On-ARNTL-shRNA3

Plasmid

#166915

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

Plasmid

#74261

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Sox17

Plasmid

#128828

Purpose

dox-dependent expression of mouse Sox17

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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FusionRed-Fibrillarin-7

Plasmid

#56118

Purpose

Localization: Nucleoli, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Fibrillarin (FBL Human)

Tags

FusionRed

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
3I91

Plasmid

#62514

Purpose

Bacterial expression plasmid for use in structural determination.

Depositor

Cheryl Arrowsmith

Article

Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

Insert

CBX8 (CBX8 Human)

Tags

6xHis and TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLenti-mitoX4-mCerulean

Plasmid

#185807

Purpose

Mitochondrial targetted (COX8) mCerulean

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mCerulean

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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