We narrowed to 3,747 results for: mak

1. pLKO-Tet-On-ARNT-shRNA3

   Plasmid

   #166912

   Purpose

   Lentivirus for inducible knockdown of human ARNT

   Depositor

   Roland Friedel

   Article

   Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

   Insert

   ARNT shRNA (ARNT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGG-GST-TEV-mCherry-NAP1 (WT)

   Plasmid

   #198036

   Purpose

   Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV and mCherry

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

   Plasmid

   #212795

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K48R/K63R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K48R/K63R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas-Cas12m-ΔZF

   Plasmid

   #192276

   Purpose

   Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   Cas12m ΔZF
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   H459A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas-dCas12m-ΔZF

   Plasmid

   #192277

   Purpose

   pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m ΔZF
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A, H549A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGG-GST-TEV-NAP1 (WT)

   Plasmid

   #198034

   Purpose

   Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV

   Expression

   Mammalian

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGST2-GST-TEV-OPTN S177D S473D

   Plasmid

   #187827

   Purpose

   Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   S177D S473D

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-mitoX4-FKBP-HA

   Plasmid

   #185658

   Purpose

   Mitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitope

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   mito-FKBP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBacMam2-DiEx-LIC-N entry vector

    Plasmid

    #111751

    Purpose

    Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. RGS-6xHis-LIN-29-pcDNA3.1-

    Plasmid

    #52515

    Purpose

    expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Lin-29 b (lin-29 Nematode)
    

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    wt (changed coding sequence in 5' (5th codon…

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mIFP-Cx26-7

    Plasmid

    #56216

    Purpose

    Localization: Gap Junctions, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Cx26 (Gjb2 Rat)
    

    Tags

    mIFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a DIO-ChRmine-oScarlet-WPRE

    Plasmid

    #183524

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    ChRmine-oScarlet
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+MLS-HyPer7

    Plasmid

    #136470

    Purpose

    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Tandem mitochondrial targeting signal of cytochro…

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid

    #229498

    Purpose

    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC 5'Untranslated region + destabilized GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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