We narrowed to 24,400 results for: lars;

1. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

   Plasmid

   #69901

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

   Plasmid

   #61762

   Purpose

   Dual Color betaGlobin reporter to study splicing kinetics in vivo

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   b-Globin
   

   Use

   Tags

   24 X MS2 hairpin cassette in 3'UTR of b-Glob…

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible Tet-ON promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide Cherry

   Plasmid

   #170510

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Inserts

   S. pyogenes sgRNA cassette
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human LFA-1 alpha

   Plasmid

   #8630

   Depositor

   Timothy Springer

   Article

   Larson et al J Cell Biol 1989 Feb;108(2):703-12.

   Insert

   LFA1 alpha (ITGAL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

6. nLbCas12a

   Plasmid

   #213745

   Purpose

   For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   nLbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LbCas12a-crRNA

   Plasmid

   #213753

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a-crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-eLACCO1.1

   Plasmid

   #167944

   Purpose

   Fluorescent biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-Nck1

    Plasmid

    #45903

    Depositor

    Louise Larose

    Article

    Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

    Insert

    Nck-1 (NCK1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSF-CjPglB-LpxE

    Plasmid

    #128389

    Purpose

    Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Inserts

    PglB oligosaccharylktransferase
    LpxE phosphatase
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and pBAD

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-Caspase-3(V266E)

    Plasmid

    #90089

    Purpose

    Expresses caspase 3 V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    V266E

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV-U6+27-Tornado-Squash-SAM-sensor 5-1

    Plasmid

    #177917

    Purpose

    Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-Squash-SAM-sensor 5-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVasa-Cre

    Plasmid

    #15885

    Depositor

    Diego Castrillon

    Article

    Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

    Insert

    mouse vasa promoter (Ddx4 Mouse)
    

    Use

    Tags

    Cre

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZW1-FCS-circRNA

    Plasmid

    #73449

    Purpose

    Expression of circular RNA

    Depositor

    Ling-Ling Chen

    Article

    Zhang et al Cell. 2014 Sep 25;159(1):134-47. doi: 10

    Insert

    POLR2A (POLR2A Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LbCas12a-D156R

    Plasmid

    #213744

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-PRMT9

    Plasmid

    #79675

    Purpose

    mammalian expression of GFP-PRMT9

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-FLEX-HM4Di-YFP

    Plasmid

    #209193

    Purpose

    Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

    Depositor

    Larry Zweifel

    Article

    Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

    Insert

    Hm4Di
    

    Use

    AAV

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHKMC1: Empty sgRNA for Cloning

    Plasmid

    #67720

    Purpose

    Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

    Depositor

    Monica Colaiacovo

    Article

    Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    PU6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

    Plasmid

    #69908

    Purpose

    Expresses a GFP circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge