Addgene

We narrowed to 20,931 results for: Tes

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pLNC Wnt-7aHA

Plasmid

#18036

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

WNT-7A (Wnt7a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
DinoIII

Plasmid

#128100

Purpose

Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

RNA complex & upstream/downstream of LHC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Inserts are potential promoters

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX2T E7 Q

Plasmid

#13648

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 Q mutant (E7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 30-37 changed from DSSEEEDE to QSSQQQQQ.

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pXJ40-HA-Merlin I S518A

Plasmid

#19700

Depositor

Filippo Giancotti

Article

Okada et al J Cell Biol. 2005 Oct 24. 171(2):361-71.

Insert

Merlin I (Nf2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 518 to Alanine

Promoter

CMV

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Hulk-ubi-lacZ-to-Green

Plasmid

#108641

Purpose

ubiquitous promoter followed by a lacZ to GFP lineage tracing line. Cryst:venusGFP

Depositor

Nadia Mercader Huber

Article

Di Donato et al Genome Res. 2016 Mar 8.

Insert

ubi-lacZ-to-GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Paxillin

Plasmid

#15212

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

Paxillin (PXN Chicken)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pJYDC2

Plasmid

#162459

Purpose

Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132D

Plasmid

#69288

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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myc-Raptor S722A/S792A

Plasmid

#18118

Depositor

Reuben Shaw

Article

Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

Insert

Raptor (RPTOR Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

S722A, S792A

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pUBQ10-YFP-GW

Plasmid

#209362

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-T7-CA150

Plasmid

#21924

Depositor

Mariano Garcia-Blanco

Article

Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

Insert

CA150 (TCERG1 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
57F03_LexA-GAD

Plasmid

#83256

Purpose

Expresses LexA-GAD under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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V1664 pLPS-3'RFP-mito - Acceptor

Plasmid

#11702

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP-mito

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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NF-155 pcDNA3

Plasmid

#197290

Purpose

Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

Depositor

Peter Brophy

Article

Collinson et al Glia. 1998 May;23(1):11-23

Insert

Pan-Neurofascin (extracellular) (Nfasc Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-LacZ10

Plasmid

#65026

Purpose

GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Use

Tags

lacZ

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV mouse Hic-5

Plasmid

#11190

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 (Tgfb1i1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-1HA

Plasmid

#18027

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1 (Wnt1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
MBP-RGG-SmBit-RGG

Plasmid

#176644

Purpose

SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-SmBit-RGG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAD

Plasmid

#64747

Purpose

Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1 (ser659ala, ser1157ala)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pALPS eGFP

Plasmid

#101323

Purpose

Encodes eGFP

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pB-actin 16 Early

Plasmid

#13711

Depositor

Karl Munger

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV 16 early region (E4 Human)

Use

Tags

Expression

Mammalian

Mutation

Incl. small part of L2

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
TSC2-3A (S1337A/S1341A/S1345A)

Plasmid

#29360

Depositor

Kunliang Guan

Article

Inoki et al Cell. 2003 Nov 26. 115(5):577-90.

Insert

TSC2 (Tsc2 Rat)

Use

Tags

Expression

Mammalian

Mutation

S1337A/S1341A/S1345A (equivalent to S1381/S1385/S…

Promoter

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
B2329_mCherry-FKBP2x

Plasmid

#215103

Purpose

Expresses mCherry-FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

mCherry-FKBP-FKBP

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-EthR

Plasmid

#51170

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

EthR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBS Notch4HA

Plasmid

#21470

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pNXRVj-HACre

Plasmid

#78407

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
aur1R (aur1 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Gly 240 to Cys

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-1HA

Plasmid

#17367

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1HA (Wnt1 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMT Draper-mCherry

Plasmid

#126667

Purpose

Inducible expression of Draper-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper mCherry

Use

Tags

Expression

Insect

Mutation

C813Y- see depositor comments below

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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