We narrowed to 9,634 results for: SUB

1. pDONR223_OR2G6_p.W149C

   Plasmid

   #81353

   Purpose

   Gateway Donor vector containing OR2G6 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2G6 (OR2G6 Human)
   

   Use

   Gateway entry vector

   Mutation

   W149C; T279N

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_OR2G6_p.N42S

   Plasmid

   #81328

   Purpose

   Gateway Donor vector containing OR2G6 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2G6 (OR2G6 Human)
   

   Use

   Gateway entry vector

   Mutation

   N42S

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_OR2T33_p.P285L

   Plasmid

   #81329

   Purpose

   Gateway Donor vector containing OR2T33 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2T33 (OR2T33 Human)
   

   Use

   Gateway entry vector

   Mutation

   P285L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_OR2T33_p.L60P

   Plasmid

   #81331

   Purpose

   Gateway Donor vector containing OR2T33 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2T33 (OR2T33 Human)
   

   Use

   Gateway entry vector

   Mutation

   L60P

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223_OR2G6_p.I177F

   Plasmid

   #81335

   Purpose

   Gateway Donor vector containing OR2G6 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2G6 (OR2G6 Human)
   

   Use

   Gateway entry vector

   Mutation

   I177F

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223_OR2T33_p.W147C

   Plasmid

   #81336

   Purpose

   Gateway Donor vector containing OR2T33 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2T33 (OR2T33 Human)
   

   Use

   Gateway entry vector

   Mutation

   W147C

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_OR2G6_p.K236M

   Plasmid

   #81342

   Purpose

   Gateway Donor vector containing OR2G6 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2G6 (OR2G6 Human)
   

   Use

   Gateway entry vector

   Mutation

   K236M

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_OR2T33_p.R120S

   Plasmid

   #81343

   Purpose

   Gateway Donor vector containing OR2T33 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2T33 (OR2T33 Human)
   

   Use

   Gateway entry vector

   Mutation

   R120S

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_OR2T33_p.W49L

   Plasmid

   #81324

   Purpose

   Gateway Donor vector containing OR2T33 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   OR2T33 (OR2T33 Human)
   

   Use

   Gateway entry vector

   Mutation

   W49L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_LCE1B_p.S42F

    Plasmid

    #81296

    Purpose

    Gateway Donor vector containing LCE1B , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    LCE1B (LCE1B Human)
    

    Use

    Gateway entry vector

    Mutation

    S42F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_RHEB_WT

    Plasmid

    #82276

    Purpose

    Gateway Donor vector containing RHEB, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB1 (RHEB Human)
    

    Use

    Gateway entry vector

    Mutation

    S86F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223_MOS_WT

    Plasmid

    #82218

    Purpose

    Gateway Donor vector containing MOS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MOS (MOS Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_OTUD6A_p.R171P

    Plasmid

    #81287

    Purpose

    Gateway Donor vector containing OTUD6A , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Gateway entry vector

    Mutation

    R171P

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223_C19orf48_p.L70F

    Plasmid

    #81288

    Purpose

    Gateway Donor vector containing C19ORF48 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    C19ORF48 (C19orf48 Human)
    

    Use

    Gateway entry vector

    Mutation

    L70F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_SPANXN4_p.P4Q

    Plasmid

    #81262

    Purpose

    Gateway Donor vector containing SPANXN4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPANXN4 (SPANXN4 Human)
    

    Use

    Gateway entry vector

    Mutation

    P4Q

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223_SPATA8_p.E18K

    Plasmid

    #81272

    Purpose

    Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPATA8 (SPATA8 Human)
    

    Use

    Gateway entry vector

    Mutation

    E18K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_RAB42_p.A61T

    Plasmid

    #81273

    Purpose

    Gateway Donor vector containing RAB42 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RAB42 (RAB42 Human)
    

    Use

    Gateway entry vector

    Mutation

    A61T

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_SPATA8_p.P83T

    Plasmid

    #81260

    Purpose

    Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPATA8 (SPATA8 Human)
    

    Use

    Gateway entry vector

    Mutation

    P83T

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Progerin-S22D-CS-pLPC

    Plasmid

    #73810

    Purpose

    Progerin mutant where Serine 22 is mutated to aspartic acid and where there is a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Aging (Albany NY). 2016 Feb;8(2):366-81.

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Progerin with substitution of cysteine in CaaX-mo…

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Plxnb2(S)-AP-His

    Plasmid

    #72003

    Purpose

    Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Jam4.3-Fc-His

    Plasmid

    #72082

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.3 (Igsf5 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Jam3-Fc-His

    Plasmid

    #72080

    Purpose

    Expresses the extracellular region of the JAM-3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam3 (Jam3 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lrrc4-AP-His

    Plasmid

    #71958

    Purpose

    Expresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4 (Lrrc4 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Jam3-AP-His

    Plasmid

    #71954

    Purpose

    Expresses the extracellular region of the JAM-3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam3 (Jam3 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Jam4.1-AP-His

    Plasmid

    #71955

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.1 (Igsf5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Jam4.3-AP-His

    Plasmid

    #71956

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.3 (Igsf5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPC-S22AProgerin-CS

    Plasmid

    #69063

    Purpose

    encodes a Progerin mutant with serine 22 mutated to an alanine and with a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Progerin with serine 22 mutated to alanine and wi…

    Promoter

    CMV

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA5-F-Med26-NTD

    Plasmid

    #49255

    Purpose

    Mammalian expression of flag-tagged Med26-NTD

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD (aa 1-339)

    Available Since

    Dec. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA5-F-Med26-R61A K62A

    Plasmid

    #49254

    Purpose

    Mammalian expression of flag-tagged human Med26 R61A K62A

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R61A K62A

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

30. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158653

    Purpose

    making transgenics expressing a UAS driven mCherry and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid

    #140982

    Purpose

    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaQ-RLuc8 (GNAQ Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR223_TNFRSF17_WT

    Plasmid

    #82263

    Purpose

    Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TNFRSF17 (TNFRSF17 Human)
    

    Use

    Gateway entry vector

    Mutation

    N81S; K86E

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR223_IRGM_WT

    Plasmid

    #82134

    Purpose

    Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRGM (IRGM Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197061

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR223_NOTCH2_WT

    Plasmid

    #82292

    Purpose

    Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NOTCH2 (NOTCH2 Human)
    

    Use

    Gateway entry vector

    Mutation

    C946Y

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid

    #140973

    Purpose

    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai1-RLuc8 (GNAI1 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223_RBM10_WT

    Plasmid

    #81958

    Purpose

    Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RBM10 (RBM10 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR223_PIK3CB_WT

    Plasmid

    #82221

    Purpose

    Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge