We narrowed to 5,211 results for: golden gate
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Plasmid#229480PurposeAdenoviral VA RNA I geneDepositorInsertVA RNA I
UseAAV and AdenoviralExpressionMammalianPromoterwt promoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-24xMS2
Plasmid#212629PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorPublicationInsertEF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-CyOFP1
Plasmid#228604PurposeMammalian expression of CyOFP1, for MuSIC barcode constructionDepositorInsertCyOFP1
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110032
Plasmid#222438PurposeLevel 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.DepositorInsertCBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110033
Plasmid#222441PurposeLevel 0, CDS module (AATG-GCTT) containing Cre recombinase.DepositorInsertCre recombinase
UseSynthetic BiologyExpressionPlantMutationBsaI/BbsI sites removed by point-mutationAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111117
Plasmid#222460PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber mosaic virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00011
Plasmid#222439PurposeLevel 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.DepositorInsertCUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110035
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW101008
Plasmid#222453PurposeLevel 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.DepositorInsertCBS4-miniDFR::OsL5::NOS
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91070
Plasmid#222463PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber Mosaic Virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-4xMS2
Plasmid#212626PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorPublicationInsertEF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
BRD4dN-mCherry-SspB
Plasmid#227387PurposeCondensate module, light-inducible oligomerization IDRDepositorInsertBRD4 IDR
UseLentiviralAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_11
Plasmid#225819PurposeTn libraries Himar variant CAM backboneDepositorPublicationTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_AtU6p_BsaI_gRNA
Plasmid#225987PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHEE-R-bar9si
Plasmid#222834PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg5A9si
Plasmid#222835PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_AF
Plasmid#217583PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_AE
Plasmid#217582PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5867
Plasmid#219751PurposepGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expressionDepositorInsertpGAP - mcitHispS - tAOX
ExpressionYeastAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
ExpressionPlantAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only