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pFGL821-8

Plasmid

#58224

Purpose

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

hygromycin B phosphotransferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pHYDuet183

Plasmid

#41685

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

PhoRadA intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSKDuet26

Plasmid

#41689

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

Tvo VMA intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(+) eGFP p6

Plasmid

#78582

Purpose

Expresses GFP p6 in mammalian cells

Depositor

Jeremy Luban

Article

Gurer et al J Virol. 2005 Jan;79(2):910-7.

Insert

eGFP p6

Expression

Mammalian

Mutation

HIV-1 HXB2 p6 with conservative mutations (G78A, …

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFGL822-8

Plasmid

#58226

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSKDuet27

Plasmid

#41698

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

Tac VMA intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pZNpF-deleted

Plasmid

#245372

Purpose

Negative control, luciferase cassette deleted

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTD68-rHUH

Plasmid

#235194

Purpose

To express rHUH-myc in bacteria

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

rHUH

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTEC15

Plasmid

#30174

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Wasabi

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pOpen-RNAse A

Plasmid

#165565

Purpose

Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

RNAse A

Use

Synthetic Biology

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTEC27

Plasmid

#30182

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

tdTomato

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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HA.PKD

Plasmid

#10808

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

L520P

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pTEC18

Plasmid

#30177

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

EBFP2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74932

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pTEC19

Plasmid

#30178

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Crimson

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pWUKI 1+2

Plasmid

#79879

Purpose

Encodes Cas1+2 and a CRISPR array

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1
Cas2
CRISPR Array

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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HA.PKD.Y463F

Plasmid

#10813

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

Y463F, inactive

Promoter

CMV

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pSKDuet16

Plasmid

#41684

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

NpuDnaE intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTEC16

Plasmid

#30175

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

mOrange2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pTEC20

Plasmid

#30179

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

mKate2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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