We narrowed to 34,848 results for: CaS;

1. pD10AH840AhCas9 (GB1041)

   Plasmid

   #68207

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. helicase cDNA

   Plasmid

   #39552

   Depositor

   Norbert Perrimon

   Article

   Eberl et al Genetics. 1997 Jul;146(3):951-63.

   Insert

   helicase (Hel25E Fly)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. RCAS (A) cPitx1 (CT#70)

   Plasmid

   #13871

   Depositor

   Cliff Tabin

   Article

   Logan et al Science. 1999 Mar 12. 283(5408):1736-9.

   Insert

   pitx1 (PITX1 Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCaSpeR-hs-taiman

   Plasmid

   #17585

   Depositor

   Denise Montell

   Article

   Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

   Insert

   taiman (tai Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pHS26_10xHis_MBP_TEV_VRER-Cas9

   Plasmid

   #244829

   Purpose

   Bacterial expression of VRER Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   VRER Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135V/G1218R/R1335E/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS16_10xHis_MBP_TEV_xPBA-Cas9

   Plasmid

   #244830

   Purpose

   Bacterial expression of xPBA Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   xPBA Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS

   Plasmid

   #244833

   Purpose

   Bacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 2xSV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS23_10xHis_MBP_TEV_SpG-Cas9

   Plasmid

   #244826

   Purpose

   Bacterial expression of SpG Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1_10xHis_MBP_TEV_WT-Cas9

   Plasmid

   #244825

   Purpose

   Bacterial expression of SpyCas9 (WT) for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS24_10xHis_MBP_TEV_SpRY-Cas9

    Plasmid

    #244827

    Purpose

    Bacterial expression of SpRY Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpRY Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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