-
Plasmid#119089PurposeBacterial expression of human phox homology (PX) domain, SNX8-PX (70-190)DepositorInsertSNX8-PX (70-190) (SNX7 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SNX15-PX (1-127)
Plasmid#119096PurposeBacterial expression of human phox homology (PX) domain, SNX15-PX (1-127)DepositorInsertSNX15-PX (1-127) (SNX15 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SNX23-PX (1178-1312)
Plasmid#119102PurposeBacterial expression of human phox homology (PX) domain, SNX23-PX (1178-1312)DepositorInsertSNX23-PX (1178-1312)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SNX24-PX (1-101)
Plasmid#119103PurposeBacterial expression of human phox homology (PX) domain, SNX24-PX (1-101)DepositorInsertSNX24-PX (1-101) (SNX24 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
PXK-PX (1-125)
Plasmid#119114PurposeBacterial expression of human phox homology (PX) domain, PXK-PX (1-125)DepositorInsertPXK-PX (1-125) (PXK Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
PI3KC2beta-PX (1348-1487)
Plasmid#119120PurposeBacterial expression of human phox homology (PX) domain, PI3KC2beta-PX (1348-1487)DepositorInsertPI3KC2beta-PX (1348-1487) (PIK3C2B Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
RICS-PX (127-255)
Plasmid#119126PurposeBacterial expression of human phox homology (PX) domain, RICS-PX (127-255)DepositorInsertRICS-PX (127-255)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseTagsExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
BK alpha subunit
Plasmid#113566Purposemouse BK channel alpha subunit expression vectorDepositorInsertKcnma1 (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
VAMP3-mCherry
Plasmid#92423PurposeExpression of VAMP3 C-terminally conjugated to mCherry.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-z ONE-GO
Plasmid#189734PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oA ONE-GO
Plasmid#189732PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationI250 (silent mutation in YFP)PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc DBC1
Plasmid#35096DepositorInsertdeleted in breast cancer 1 (CCAR2 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-13 ONE-GO
Plasmid#189740PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationL342M (present in YFP but no functional consequen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-12 ONE-GO
Plasmid#189739PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-CAD
Plasmid#46238PurposeFor expression of HA and FLAG-tagged murine CAD in mammalian cellsDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-13 ONE-GO
Plasmid#204337PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-13 ONE-GO (GNA13 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEND-303_Cacnes_tetR
Plasmid#225618PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-304_Cacnes_tetR
Plasmid#225619PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-375_Cacnes_phlF
Plasmid#225620PurposeC. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.DepositorInsertPhlF
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9-msa
Plasmid#164833PurposemiCas9-msa fusion protein expressing plasmidDepositorInsertmiCas9-msa
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationcontains mSA sequence range is 5106-5447 and Brex…PromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Budding Yeast, Zebrafish, Neurospora crassa)
UseZebrafish expressionTagsExpressionMutationPromoterhsp70lAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gluc-LHCGR
Plasmid#221781PurposeMeasurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGRDepositorInsertLHCGR (LHCGR Human)
UseTagsGaussia Luciferase and HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-acta1:QFGal4-SV40pA
Plasmid#155121PurposeTol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertacta1:QFGal4
UseZebrafish expressionTagsExpressionMutationPromoteracta1Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash6
Plasmid#74134PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorUseTags2xSTIIExpressionInsectMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only