Addgene

We narrowed to 2,963 results for: NAK

Showing: 2541 - 2560 of 2963 results

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PB-TRE3G-TP53-CCRB

Plasmid

#236773

Purpose

A piggybac-based cloning vector containing TRE3G promoter-driven full-length TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

TP53 (TP53 Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G promoter and TRE3G promoter, CAG promoter

Available since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)

Plasmid

#236419

Purpose

transient overexpression of EMSY in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

EMSY (2210018M11Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)

Plasmid

#236420

Purpose

transient overexpression of EMSY in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

EMSY (EMSY Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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SB-CAG-mCherry-P2A-EIF3D-NN-IP

Plasmid

#236771

Purpose

A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528N/S529N-IRES-Pac.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

EIF3D (EIF3D Human)

Use

Sleeping beauty

Tags

Expression

Mammalian

Mutation

changed Serine 528 to Asparagine and Serine 529 t…

Promoter

CAG promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN_4A

Plasmid

#227435

Purpose

Expression of the R-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN 4A (Pten Rat)

Use

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CAG_G-PTEN

Plasmid

#227436

Purpose

Expresses the G-PTEN sensor under a CAG promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)

Use

Tags

mEGFP, and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN

Plasmid

#227433

Purpose

Expresses the R-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN (Pten Rat)

Use

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN_4A

Plasmid

#227434

Purpose

Expresses the G-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN 4A (Pten Rat)

Use

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

4A mutation.

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcs2-cDELE1(142-515)-sumo

Plasmid

#226107

Purpose

cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

DELE1 (DELE1 Human)

Use

Tags

Sumo

Expression

Mammalian

Mutation

encodes only residues 142-515 of DELE1 (represent…

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2-MTS-cox8A-GFP-IRES-mCherry

Plasmid

#226103

Purpose

Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

COX8A (COX8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

encodes only for mitochondrial targeting sequence…

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2-cDELE1(142-515)-GFP-IRES-mCherry

Plasmid

#226102

Purpose

cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

DELE1 (DELE1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

only encodes for residues 142-515 of DELE1 corres…

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Expression

Mutation

Promoter

U6

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del18-197)-FLAG

Plasmid

#204539

Purpose

Mammalian expression of human CALR ins5 (del18-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del18-197)-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

ins5 (del18-197)

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del198-430)-FLAG

Plasmid

#204538

Purpose

Mammalian expression of human CALR ins5 (del198-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-430)-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

ins5 (del198-430)

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del367-430)-FLAG

Plasmid

#204536

Purpose

Mammalian expression of human CALR ins5 (del367-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del367-430)-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

ins5 (del367-430)

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del309-430)-FLAG

Plasmid

#204537

Purpose

Mammalian expression of human CALR ins5 (del309-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del309-430)-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

ins5 (del309-430)

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del1-308)-FLAG

Plasmid

#204535

Purpose

Mammalian expression of human CALR ins5 (del1-308)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-308)-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

ins5 (del1-308)

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del198-308)-FLAG

Plasmid

#204540

Purpose

Mammalian expression of human CALR ins5 (del198-308)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-308)-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

ins5 (del198-308)

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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