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We narrowed to 4,373 results for: p2a

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Showing: 2541 - 2560 of 4373 results

  4. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  5. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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  7. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235307
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235307-plasmid-map-sequence-id-472801

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  13. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235308
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235308-plasmid-map-sequence-id-472753

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  15. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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Showing: 2541 - 2560 of 4373 results