We narrowed to 14,886 results for: pET

1. pET28-eIF4E-TwStrep

   Plasmid

   #208638

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

   Depositor

   Quanfu Ma

   Insert

   human eIF4E (ENST00000450253) (EIF4E Human)
   

   Use

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-RPL3-Twstrep

   Plasmid

   #208623

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) (RPL3 Human)
   

   Use

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-NUbo-E3(63)-His6

   Plasmid

   #212717

   Purpose

   Bacterial expression of NUbo-E3(63)-His6

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(63)
   

   Use

   Tags

   6xHis and NUbo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24a-NUbo-E3(48)-His6

   Plasmid

   #212714

   Purpose

   Bacterial expression of NUbo-E3(48)-His6

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48)
   

   Use

   Tags

   6xHis and NUbo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-hXRCC1pd-HIS

   Plasmid

   #206036

   Purpose

   Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   hXRCC1
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

   Promoter

   T7

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30b jGCaMP8f L27A

   Plasmid

   #204138

   Purpose

   Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Use

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   T7

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-15b-aδNb072

   Plasmid

   #204627

   Purpose

   Expresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.

   Depositor

   James McDonnell

   Article

   Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

   Insert

   aδNb072
   

   Use

   Tags

   His6 and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETSyt1C2AB(80-421)msGFP

   Plasmid

   #209009

   Purpose

   Recombinant protein expression of truncated syt1 tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-421 only

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETmsGFPSyt1(80 JuxtaK-142)

   Plasmid

   #209013

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis-msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETSUMOSyt1C2AB(80 JuxtaK-421)

    Plasmid

    #209008

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETSUMOSyt1C2AB(143-421)

    Plasmid

    #209007

    Purpose

    Recombinant protein expression of truncated syt1 construct

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 143-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pet28b-His_RAD23A-UBA2

    Plasmid

    #201550

    Purpose

    Expresses UBA2 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 311-363 of DNA polymerase i…

    Promoter

    T7

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET (SUMO)-Syt9

    Plasmid

    #195713

    Purpose

    Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Rat)
    

    Use

    Tags

    GST-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-GFP-mSOX17

    Plasmid

    #206379

    Purpose

    Expresses EGFP fused mouse SOX17 in bacteria, for protein production.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox17 (Sox17 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28 mePOU-POU

    Plasmid

    #206392

    Purpose

    Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    ePOU
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC-cUnaG-LPETG

    Plasmid

    #207635

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 mOct4-POU

    Plasmid

    #206393

    Purpose

    Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-GFP-mSOX17FNV

    Plasmid

    #206380

    Purpose

    Expresses EGFP fused mouse SOX17FNV in bacteria, for protein production.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox17FNV (Sox17 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-GFP-miniSOX

    Plasmid

    #206381

    Purpose

    Expresses EGFP fused miniSOX in bacteria, for protein production.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    miniSOX
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET3-ARF1-His6

    Plasmid

    #201883

    Purpose

    Used for bacterial expression of human protein ARF1

    Depositor

    Richard Kahn

    Insert

    ARF2
    

    Use

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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