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pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_TEV_mVenus_FG

Plasmid

#240673

Purpose

Expression plasmid for mCherry-TEV-mVenus-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_2xStrepII_FG

Plasmid

#240675

Purpose

Expression plasmid for TEV-2xStrepII-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-2xStrepII

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_TEV_mVenus_Hyg

Plasmid

#240653

Purpose

Expression plasmid for mCherry-TEV-mVenus-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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HDM-tat1b

Plasmid

#204154

Purpose

Lentivirus helper plasmid coding for Tat

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

tat

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1m

Plasmid

#190474

Purpose

Express the medium-affinity histamine sensor GRAB_HA1m in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-hNINJ1-GFP

Plasmid

#208779

Purpose

Inducibly expresses human NINJ1 tagged with GFP

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (NINJ1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Tags

mEos2

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1h

Plasmid

#190473

Purpose

Express the high-affinity histamine sensor GRAB_HA1h in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1h (high affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-FLAG

Plasmid

#183511

Purpose

Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Type

Empty backbone

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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SpyTag-sfGFP

Plasmid

#184226

Purpose

Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpyTag-sfGFP

Tags

His6, SpyTag, and TEV protease cleavage site

Expression

Bacterial

Promoter

T5

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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B52_puro_empty_gRNA

Plasmid

#197557

Purpose

sites for cloning two gRNA with puromycin cassette

Depositor

Darius Bägli

Type

Empty backbone

Use

Empty grna backbone with puromycin resistance gene

Expression

Mammalian

Promoter

U6 for gRNA, CMV for puromycin resistance.

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mem-TurboID

Plasmid

#149409

Purpose

Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG roGFP2-mito

Plasmid

#168509

Purpose

Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-roGFP2

Tags

roGFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 mCherry

Plasmid

#105010

Purpose

Excitatory presynaptic marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-mNINJ1-GFP

Plasmid

#208775

Purpose

Inducibly expresses mouse NINJ1 tagged with GFP

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

mNINJ1 (Ninj1 Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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