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pSH100 (YCplac33 MET25pro MCP-mCherry)

Plasmid

#45930

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

MCP-mCherry

Tags

mCherry

Expression

Yeast

Promoter

Met25

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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tetO-NKX2.5

Plasmid

#46029

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

NKX2.5 (NKX2-5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-D

Plasmid

#38133

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-D (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-miR-9 sponge

Plasmid

#25040

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 sponge

Use

Retroviral

Tags

d2EGFP

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV4-3HA

Plasmid

#24165

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Type

Empty backbone

Tags

3xHA

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pENTRY-GFP

Plasmid

#15301

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

hrGFP

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac1 INI1

Plasmid

#1953

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

INI1 (SMARCB1 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pACgp67B-Her2

Plasmid

#10794

Depositor

Judy Lieberman

Article

Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

Insert

ErbB2 ScFv (ERBB2 Human)

Tags

6His

Expression

Insect

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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Cdk4-DN-HA

Plasmid

#1877

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pAM1

Plasmid

#51401

Purpose

Expresses truncated version of NLL-MetRS from endogenous MetG promoter

Depositor

David Tirrell

Article

Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Insert

MetRS (metG E coli)

Mutation

L13N, Y260L, H301L; Please note that a truncated …

Promoter

MetG

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Expression

Worm

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-hMLH1dn

Plasmid

#222997

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Deletion of residues 754-756 in MLH1 as described…

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS1

Plasmid

#185136

Purpose

non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-8XGTIIC-DsRED-DD

Plasmid

#115798

Purpose

lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP responsive promoter (YAP1 Human)

Use

Lentiviral

Tags

DsRED-DHFR

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.EGFP

Plasmid

#100047

Purpose

AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pPapi

Plasmid

#96921

Purpose

expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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