We narrowed to 3,447 results for: arl

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pPI468

Plasmid

#113207

Purpose

integrating expression vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Tags

mScarlet

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRD403

Plasmid

#163108

Purpose

Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mScarlet-I_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -0

Plasmid

#173889

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

Plasmid

#173890

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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t1-400

Plasmid

#166577

Purpose

For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

Tln1 Head (1-400) (TLN1 Human)

Tags

His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

Expression

Bacterial

Mutation

residues 1-400 only

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMini-CAAGs::RFP-DR48

Plasmid

#132640

Purpose

Fluorescent reporter to quantify elicitation of the strand annealing mediated repair pathway

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

mRFP

Expression

Mammalian

Mutation

Contains a 92bp insertion interrupting the open …

Promoter

Chicken B-actin

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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ZE36 Nlk1(C387Y)-pCS2+

Plasmid

#17077

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Mutation

C387Y. Inactive mutant of NLK1.

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE37 Nlk1(K117M)-pCS2+

Plasmid

#17078

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Mutation

K117M. Kinase dead mutant of NLK1.

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T 88

Plasmid

#10690

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 88 (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

Contains only the N-termial 88 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-hARFRP1(T31N)-HA

Plasmid

#206401

Purpose

Mammalian expression of hARFRP1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(Q79L)-HA

Plasmid

#206400

Purpose

Mammalian expression of hARFRP1(Q79L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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MZB220

Plasmid

#221771

Purpose

Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arl1 (ARL1 Human)

Tags

CPD

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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TINGL-RR pDRF1

Plasmid

#226435

Purpose

Expresses TINGL-RR (mTq2-based glucose sensor - O2A - ymScarletI) in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2026 Feb 2. doi: 10.1021/acsse

Insert

TINGL-O2A-ymScarletI

Expression

Yeast

Promoter

PMA1

Available Since

Dec. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCSIIpuro-Necl5-ScNeo

Plasmid

#209901

Purpose

To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Necl5-ScNeo (Pvr Mouse)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔCy-mCherry-Puro

Plasmid

#193764

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔCy-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'3'short

Plasmid

#129750

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM1-EGFP

Plasmid

#107794

Purpose

donor vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM1-eGFP (DNM1 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Expression

Bacterial and Yeast

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCAS9n(BB)-2A-puro DNM1, A

Plasmid

#107795

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Expression

Mammalian

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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