We narrowed to 3,092 results for: pcdna3 1

1. mVenus-P75ECD-VfAU1-LOV_458

   Plasmid

   #63634

   Purpose

   Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by mVenus, the extracellular and transmembrane domain of p75NTR and a restriction site

   Depositor

   Harald Janovjak

   Insert

   mVenus-P75ECD-VfAU1-LOV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. hMEF2A WT

   Plasmid

   #118354

   Purpose

   This plasmid expresses human MEF2A-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniABEMax V82G wt

   Plasmid

   #135362

   Purpose

   Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax nSpyCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA SF-HERC2 F1

   Plasmid

   #55784

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pTS2380-Tier1-PhCMV-TM

   Plasmid

   #169485

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmyc-GFP-TNRC6B-(997-1084)-NES-mut

   Plasmid

   #42025

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6B (TNRC6B Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa997-1084, L1009A, F1013A, M1016A, L1018A

   Promoter

   CMV

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyCas9-AIDx

   Plasmid

   #135343

   Purpose

   Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABEMax nSaCas9

   Plasmid

   #135364

   Purpose

   Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax nSaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. hTPST2

   Plasmid

   #11253

   Depositor

   Hyeryun Choe

   Article

   Choe et al Cell. 2003 Jul 25. 114(2):161-70.

   Insert

   TPST2 (TPST2 Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. hTPST1

    Plasmid

    #11252

    Depositor

    Hyeryun Choe

    Article

    Choe et al Cell. 2003 Jul 25. 114(2):161-70.

    Insert

    TPST1 (TPST1 Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

11. SPLICS Mt-ER- PM Long P2A

    Plasmid

    #164109

    Purpose

    Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM-Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPLICS Mt-ER- PM Short P2A

    Plasmid

    #164110

    Purpose

    Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM- Mt-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Slo1/BKAlpha maxi-K+ channel scFv [L6/60]

    Plasmid

    #199421

    Purpose

    Mammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Diacylglycerol Kinase Zeta

    Plasmid

    #85454

    Purpose

    expression of Diacylglycerol Kinase Zeta

    Depositor

    Stephen Gee

    Article

    Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

    Insert

    Diacylglycerol Kinase Zeta (DGKZ Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid

    #132554

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJB180

    Plasmid

    #235737

    Purpose

    Expression of mCherry- human RAB1A Q70L in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Q70L

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

    Plasmid

    #153308

    Purpose

    Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

    Depositor

    Marcel Bruchez

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    IgKappa-myc-dL5-2XG4S-GPI
    

    Use

    Tags

    myc-dL5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1029-AIDx-CP

    Plasmid

    #135341

    Purpose

    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1029 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT-C34-H

    Plasmid

    #223966

    Purpose

    Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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