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pAc5.1A-Dm-miR-14

Plasmid

#22763

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 14 (mir-14 primary transcript, Fly)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-315

Plasmid

#22765

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro-RNA 315 (mir-315 primary transcript, Fly)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215329

Purpose

Lentiviral mammalian vector with firefly luciferase gene with no upstream promoter sequence; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Publication

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

No Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Available since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86022

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Publication

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Publication

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Tags

Strep II-MBP

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2793

Plasmid

#64043

Purpose

pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter and none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDC184

Plasmid

#70055

Purpose

Co-expresses Syt1-SNARE37aa-linker complex with p045

Depositor

Axel Brunger

Publication

Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

Inserts

His tagged Synaptobrevin 28-89
Syntaxin 191-256

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV10-Flag-Fbxl21

Plasmid

#48752

Purpose

Expression of FLAG-tagged Fbxl21

Depositor

Joseph Takahashi

Publication

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Publication

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Publication

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
EF1a_Puro_Telo_v2

Plasmid

#195139

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Publication

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Expression

Mammalian

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-Control-5'UTR

Plasmid

#184637

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

Depositor

Hao Wu

Publication

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pETM6-G6-vioABE

Plasmid

#73438

Purpose

Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

Depositor

Mattheos Koffas

Publication

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 (orthogonal T7-lac variant)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 I367T IRES mRuby3

Plasmid

#170642

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Publication

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Mutation

I367T

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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G421C_BattB(GA)_mCherry

Plasmid

#200633

Purpose

Strong mCherry fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad

Depositor

Kenneth Matreyek

Publication

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

mCherry

Expression

Mammalian

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K840R

Plasmid

#204418

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Publication

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 840 to Arginine (K840R)

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K1097R

Plasmid

#204417

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Publication

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 1097 to Arginine (K1097R)

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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