Addgene

We narrowed to 761 results for: NEB

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GFP H2B SL2

Plasmid

#227718

Purpose

Plasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp Resistant

Depositor

Haosheng Sun, Oliver Hobert

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

GFP H2B SL2 (nlp-51 Nematode)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Haosheng Sun, Oliver Hobert

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SL2 GFP H2B

Plasmid

#227717

Purpose

Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Haosheng Sun, Oliver Hobert

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

SL2 GFP H2B (nlp-51 Nematode)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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T2A 3xNLS GFP

Plasmid

#227721

Purpose

Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Haosheng Sun, Oliver Hobert

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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MDH1_KW

Plasmid

#224145

Purpose

IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-1 (F46E10.10 Nematode)

Use

Tags

Ala-intein-CBD

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Strep-TGT-His

Plasmid

#206495

Purpose

Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

Depositor

Neal Devaraj

Article

Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

Insert

E. coli tRNA Guanine Transglycosylase

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTYB2-eIF4B-A130C

Plasmid

#37227

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Use

Tags

Expression

Bacterial

Mutation

A130C

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Use

Tags

MBP-6xHis-NLS

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9-VP64

Plasmid

#68417

Purpose

Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG and VP64

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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JD94

Plasmid

#229479

Purpose

CMV promoter driving expression of adenoviral E2A gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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