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Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-P(11)4
Plasmid#185780PurposeEncodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-P(11)4
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-λN-βLac
Plasmid#162039PurposeExpresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)DepositorInsertλN domain of the λ phage and a β-lactamase fusion protein
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P to A)
Plasmid#62069PurposeExpression of human p53 (1-73) (all P to A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; all Prolines changed …PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-AvrPWT4
Plasmid#237522PurposeRecombinant protein expressionDepositorInsertAvrPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-RWT4Ca
Plasmid#237519PurposeRecombinant protein expressionDepositorInsertRwt4Ca
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-KD
Plasmid#237520PurposeRecombinant protein expressionDepositorInsertRwt4_kinase
UseTagsExpressionBacterialMutationKinase region of RWT4PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-VirPWT4
Plasmid#237523PurposeRecombinant protein expressionDepositorInsertVirPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-CC-S2
Plasmid#233243PurposeBacterial expression of Avi-CC-S2DepositorInsertAviTag-CC-S2
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CsHPAD-AE
Plasmid#226982PurposeExpressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coliDepositorInsertCsHPAD-AE
UseTagsHis6 (on backbone)ExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD-AE
Plasmid#226968PurposeExpressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coliDepositorInsertOuIAD-AE
UseTagsHis6 (on backbone)ExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_lemur
Plasmid#214332PurposeProtein overexpression in bacteriaDepositorInsertIL18
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_sheep
Plasmid#214335PurposeProtein overexpression in bacteriaDepositorInsertIL18
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_rat
Plasmid#214333PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Rat)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_rabbit
Plasmid#214334PurposeProtein overexpression in bacteriaDepositorInsertIL18
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_dog
Plasmid#214336PurposeProtein overexpression in bacteriaDepositorInsertIL18
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-WM
Plasmid#208798PurposeXenopus laevis His6-Myc-DONSON (W234L, M463T)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationW234L; M463TPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-3A
Plasmid#208800PurposeXenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationV473A; R476A; Y481APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only