Addgene

Showing: 241 - 260 of 9564 results

Results per page:

Sort results by:

pAc5.1B-lambdaN-HA-DmEDC3-FDF-ADA_C

Plasmid

#146037

Purpose

Insect Expression of DmEDC3-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-FDF-ADA (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

Plasmid

#146039

Purpose

Insect Expression of DmTral-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDF-ADA (tral Fly)

Use

Tags

Expression

Insect

Mutation

FDF to ADA (407-409) mutation. Additionally, thre…

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-TEV-Gly-RadA∆C46

Plasmid

#128702

Purpose

Expresses PhoRadA intein split at residue 126 from the N-terminal

Depositor

Sharon Rozovsky

Article

Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

Insert

RadA

Use

Tags

Hisx6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MoClo adapted pUC57-Kan Level 2

Plasmid

#109218

Purpose

Non binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

Depositor

Elena Caro Bernat

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDNA3-TADA1-HFD(140-230)

Plasmid

#105984

Purpose

transient express TADA1-HFD fragment in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1-HFD (140-230) (Tada1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

Plasmid

#103864

Purpose

dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GSGGGGS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-6xHis-MBP-TEV site-TadA8r

Plasmid

#208318

Purpose

Overxpression MBP-TadA8r

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

Plasmid

#191172

Purpose

Human ADAR1, p150 isoform in plant-expressable format.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)

Use

Tags

3FLAG and HIS

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL

Plasmid

#192068

Purpose

Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Ctip2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
All-in-one split-ADAR2(E488Q, N496F)

Plasmid

#170148

Purpose

AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

Plasmid

#189562

Purpose

Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contains aa 1-213

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Promoter

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
pNtCp-rbcL-PHLS-GPPS-aadA-accD

Plasmid

#52471

Purpose

Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum

Depositor

Anastasios Melis

Insert

rbcL-PHLS-GPPS-aadA-accD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EW446 CMV-TO-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#236125

Purpose

Plasmid encoding free ADAR2dd under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ADAR2dd (ADARB1 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A

Promoter

CMV promoter with two TetR binding sites

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ088 (IVT) CMV-TO-T7-CCR6-V2-ADAR2dd-hybridUTR3

Plasmid

#233421

Purpose

IVT template for CCR6-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-CCR6-V2-ADAR2dd-hybridUTR3

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ387 (IVT) CMV-TO-T7-AVPR2-V2-ADAR2dd-hybridUTR3

Plasmid

#233423

Purpose

IVT template for AVPR2-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-AVPR2-V2-ADAR2dd-hybridUTR3

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LSM101 CMV-TO-ADAR2dd(E488Q,T501A)-MCP

Plasmid

#233432

Purpose

ADAR2dd(E488Q,T501A)-MCP (positive control that fuses ADAR2dd and MCP on the same protein)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-tdMCP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge