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Plasmid#21825DepositorInsertabu-1 promoter and coding region (abu-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
GCN2-KD1-pGEX4T-1
Plasmid#21876DepositorInsertGCN2 (Eif2ak4 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-485-3p
Plasmid#103553PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-485-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-485-3p target (MIR485 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW102
Plasmid#115945PurposeORF insert for replicon MoCloDepositorInsertLvl0 DDd-L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-146a-3p
Plasmid#103247PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-146a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-146a-3p target (MIR146A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-3p
Plasmid#103145PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-3p target (MIRLET7A3 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Src42a
Plasmid#126673PurposeExpression of GST-Src42a for purification from E. coliDepositorInsertSrc42a
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p4XBRE-SBE-SV40-GL3
Plasmid#67811Purposeluciferase reporter with mouse Id1 promoter fragmentsDepositorInsertId1 (Id1 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterId1/SV40Available sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC+CKK
Plasmid#59041Purposefor baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cellsDepositorInsertCAMSAP3 CC+CKK domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1 S449A/T450A/S454A
Plasmid#19847DepositorInsertMKL1 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGExpressionMutationchanged Serine 449, Threonine 450 and Serine 454 …PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(22/19), AB(monomer), wt
Plasmid#22380DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsHis and mycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-mCherry
Plasmid#126667PurposeInducible expression of Draper-mCherry in S2 cellsDepositorInsertDraper mCherry
UseTagsExpressionInsectMutationC813Y- see depositor comments belowPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
aCE121
Plasmid#204492PurposeCadherin circuitDepositorInsertsEBFP2
Cdh6-mKate2
Cdh1-EYFP
UseSynthetic BiologyTags2AExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(RK)
Plasmid#27136DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationArginines in the SH2 domains mutated to lysines (…PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3
Plasmid#21800DepositorInsertPSMD14 (Psmd14 Mouse)
UseTagsGST and TEVExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S454A
Plasmid#19844DepositorInsertMKL1 S454A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 454 to AlaninePromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A
Plasmid#74606Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103A, W120APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A
Plasmid#74605Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only