Addgene

Showing: 241 - 260 of 11120 results

Results per page:

Sort results by:

pLV-mScarlet3-CAAX-IRES-Puro

Plasmid

#222639

Purpose

Live-cell plasma membrane marker

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mScarlet3-CAAX

Use

Lentiviral

Tags

mScarlet3

Expression

Mutation

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse ULK1 (Ulk1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse ULK2 (Ulk2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pLV-RhoB Sensor-IRES-Blast

Plasmid

#222633

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

RhoB Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-V5-DGCR8-mDRBD1&2

Plasmid

#51385

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S6…

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-V5-DGCR8-mDRBD1

Plasmid

#51384

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD1 mutant (A568K, A569K)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-V5-DGCR8-mDRBD2

Plasmid

#51386

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD2 mutant (A676K, S677K)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
NmMetQ

Plasmid

#172111

Purpose

Expression of Methionine Binding Protein (MetQ) with signal sequence

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Insert

Methionine Binding Protein

Use

Tags

10x Histidine Tag and N.meningitidis signal seque…

Expression

Bacterial

Mutation

Changed Proline 22 to Alanine

Promoter

T7 Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-miRFP680-NES-p2A-dTomato-2xrGBD

Plasmid

#222638

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-dTomato-2xrGBD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast

Plasmid

#222634

Purpose

Rho reporter, sensor dead

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

RhoB Sensor (PKN-L59Q)

Use

Lentiviral

Tags

Expression

Mutation

PKN L59Q

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-mClover3

Plasmid

#214922

Purpose

expression vector control - contitutive expression of mClover3 alone

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

mClover3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-mRuby3

Plasmid

#214923

Purpose

expression vector control - contitutive expression of mRuby33 alone

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

mRuby3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X2-hDrp1(518-736)-Flag

Plasmid

#37244

Depositor

David Kashatus, Christopher Counter

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Drp1 (DNM1L Human)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

contains AA 517-end

Promoter

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only
lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Phil Sharp, Feng Zhang

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mRuby3-MLC-IRES-Neo

Plasmid

#222635

Purpose

Reporter for activated NM2

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mRuby3-MLC (MYL9 Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-SRRD-mRuby3

Plasmid

#214920

Purpose

inducible expression of SRRD tagged on C-terminus with mRuby3

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-SRRD-noTag (in pLEX_307)

Plasmid

#214919

Purpose

constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NmMetQ C20A

Plasmid

#172112

Purpose

Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Insert

Methionine Binding Protein

Use

Tags

10x Histidine Tag and N.meningitidis signal seque…

Expression

Bacterial

Mutation

Changed Proline 22 to Alanine on MetQ and Cystein…

Promoter

T7 Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TREtight-mTagBFP2-B19G

Plasmid

#100799

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge