Addgene

Showing: 241 - 259 of 259 results

Results per page:

Sort results by:

pLX304 PCSK5 (H516P)-V5 blast

Plasmid

#83103

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Histidine 516 to Proline

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shGDF11 #2

Plasmid

#83084

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX302 PCSK5 (T288P)-V5 puro

Plasmid

#98709

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Promoter

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO shMKP7.v2 puro

Plasmid

#87795

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2 3XFLAG-EGFP-NLS

Plasmid

#87778

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Tags

3xFLAG and NLS

Expression

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

Plasmid

#87777

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7-EE

Use

Tags

3xFLAG and NLS

Expression

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Use

Tags

3xFLAG and NLS

Expression

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

Plasmid

#72924

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Serine#37 changed to Alanine;Silent mutations mad…

Promoter

TRE tight

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

Plasmid

#72609

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed threonine34 to alanine

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

Plasmid

#72610

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33 to alanine

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pEN_TT 3XFLAG-S37A-GATA6-3XAU1

Plasmid

#72613

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Changed serine37 to alanine

Promoter

TRE tight promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

Plasmid

#72617

Purpose

Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

Serine#37 changed to Alanine;Silent mutations mad…

Promoter

TRE tight

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

Plasmid

#72611

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33, threonine34 and serine37 to ala…

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRC2_3xflag_Luciferase

Plasmid

#136519

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

Luciferase

Use

Entry vector for gateway cloning

Tags

3x FLAG

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2_BirA_NRF2

Plasmid

#136524

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2_p53(R280K)_V5

Plasmid

#136525

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53(R280K) (TP53 Human)

Use

Entry vector for gateway cloning

Tags

V5

Expression

Mutation

p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2_3xflag_CHEK2

Plasmid

#136526

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CHEK2 (CHEK2 Human)

Use

Entry vector for gateway cloning

Tags

3x FLAG

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO.1_puro_shJUND #2

Plasmid

#136583

Purpose

Expresses an inducible short hairpin targeting human JUND sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shJUNDv2 (JUND Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXSN_HPV16E7∆DLYC

Plasmid

#136588

Purpose

Retroviral vector for expression of RB-binding mutant of HPVE7

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

HPV16E7∆DLYC

Use

Retroviral

Tags

Expression

Mutation

del aa 21-24, full length is 1-98

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge