We narrowed to 251 results for: tet off

1. Strep-HA-VCF1 WT

   Plasmid

   #223020

   Purpose

   Expression of Strep-HA-tagged VCF1 in mammalian cells.

   Depositor

   Niels Mailand

   Article

   Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

   Insert

   VCF1 (VCF1 Human)
   

   Use

   Tags

   Strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Desmoplakin I tension sensor (F40-based)

   Plasmid

   #119186

   Purpose

   The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Desmoplakin I no force control (F40-based)

   Plasmid

   #119187

   Purpose

   The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   truncation after aa1945

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-VCF1 N167A

   Plasmid

   #223019

   Purpose

   Expression of GFP-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

   Depositor

   Niels Mailand

   Article

   Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

   Insert

   VCF1 (VCF1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N167A

   Promoter

   CMV/TetO2

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Strep-HA-VCF1 N167A

   Plasmid

   #223021

   Purpose

   Expression of Strep-HA-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

   Depositor

   Niels Mailand

   Article

   Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

   Insert

   VCF1 (VCF1 Human)
   

   Use

   Tags

   Strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Desmoplakin I acceptor only control (F40-based tension sensor)

   Plasmid

   #119189

   Purpose

   The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Desmoplakin I affinity mutant (F40-based tension sensor)

   Plasmid

   #119190

   Purpose

   The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Desmoplakin I donor only control (F40-based tension sensor)

   Plasmid

   #119188

   Purpose

   The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EP004: pAAV TRE::GEM7-HaloTag

   Plasmid

   #209304

   Purpose

   Doxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice

   Depositor

   Adam Cohen

   Insert

   GEM7
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-DEST (JDW 1205)

    Plasmid

    #229821

    Purpose

    A PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

    Plasmid

    #170488

    Purpose

    SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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