164,577 results

1. pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

   Plasmid

   #117661

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-alphaf(I)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-NFAT1(4-460)-GFP

   Plasmid

   #11107

   Purpose

   Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Use

   Tags

   3x HA and GFP

   Expression

   Mammalian

   Mutation

   Contains amino acids 4-460 of NFAT1; L78P compare…

   Promoter

   CMV

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN1-PinkyCaMP

   Plasmid

   #232861

   Purpose

   A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Beta-2-adrenergic receptor-YFP

   Plasmid

   #67146

   Purpose

   Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

   Plasmid

   #162280

   Purpose

   Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

   Depositor

   Al George

   Article

   DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

   Insert

   SCN8A Variant 1, stabilized (SCN8A Human)
   

   Use

   Tags

   IRES-mScarlet

   Expression

   Mammalian

   Mutation

   Modified human HSPA5 intron 4 inserted after bp23…

   Promoter

   CMV

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLM1110_LEU2

   Plasmid

   #168460

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   mRFP1
   

   Use

   Synthetic Biology; Yeast assembly vector for big-…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a MDHP_YEAST

   Plasmid

   #204233

   Purpose

   Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHP_YEAST (MDH3 Budding Yeast)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   The last 3 C-terminal residues (SKL) are the pero…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

   Plasmid

   #194457

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-CD19 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCL-c-MNDU3-X

    Plasmid

    #81071

    Purpose

    Empty lentiviral backbone with the MNDU3 promoter

    Depositor

    Donald Kohn

    Article

    Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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