We narrowed to 6,038 results for: WUS;

1. AAV_Actb MMEJ donor

   Plasmid

   #97315

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIR-21 Reporter

   Plasmid

   #71870

   Purpose

   Mammalian expression vector for the analysis of miR-21 activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-21
   

   Use

   Tags

   firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Tag2B-E2F2

   Plasmid

   #202521

   Purpose

   Expressing human E2F2 protein with FLAG-tag

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   E2F2 (E2F2 Human)
   

   Use

   Tags

   FLAG-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265F2

   Plasmid

   #164722

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)NG-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. α-catenin ECFP-YPET FRET ΔF-actin binding site

   Plasmid

   #198245

   Purpose

   Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Deleted F-actin binding site on a-catenin (849-90…

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-K-GECO1

   Plasmid

   #105864

   Purpose

   Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Use

   Tags

   6-His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLNCX2-OptoTGFBRs

   Plasmid

   #118965

   Purpose

   Retroviral expression OptoTGFBRs for transient or stable constitutive expression

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
   

   Use

   Retroviral

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HTRA1_PDZ_1

   Plasmid

   #103912

   Purpose

   Protein expression and purification of HTRA1 PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   HTRA1 (HTRA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. DLG4_PDZ_3

   Plasmid

   #103953

   Purpose

   Protein expression and purification of PSD95 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   DLG4 (DLG4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR v2-sgNCKAP1-2

    Plasmid

    #210134

    Purpose

    knock out NCKAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Nck-associated protein 1 (NCKAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2-sgNCKAP1-1

    Plasmid

    #210133

    Purpose

    knock out NCKAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Nck-associated protein 1 (NCKAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTMB400_ZFcharm_Prnp_DPM

    Plasmid

    #220847

    Purpose

    AAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTMB399_ZFcharm_Prnp_SPM

    Plasmid

    #220846

    Purpose

    AAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTMB340_ZFcharm_Kv1_Prnp_DPM

    Plasmid

    #220843

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-ACSL4-FL

    Plasmid

    #202515

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p23-NES-PspCas13b-msfGFP-NES-Flag

    Plasmid

    #165071

    Purpose

    overexpression of PspCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. WT-β3

    Plasmid

    #166579

    Purpose

    For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

    Depositor

    Vesa Hytönen

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

    Insert

    β3 (Itgb3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-FH-AGO2-R438E

    Plasmid

    #91984

    Purpose

    Expresses FLAG-HA-AGO2 (R438E)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    R438E

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pRK5-HA-Ubiquitin-K27R-K29R

    Plasmid

    #121156

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R and K29R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K27 and K29 mutated to Rs

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AFDN_PDZ_1

    Plasmid

    #103938

    Purpose

    Protein expression and purification of TRITHORAX PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    AFDN (AFDN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains AA983-1110 of AFDN

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FUW-TetO-Eomes

    Plasmid

    #128825

    Purpose

    dox-dependent expression of mouse Eomes

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Shorter isoform

    Promoter

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLJM1-FH-AGO2-K525E

    Plasmid

    #91985

    Purpose

    Expresses FLAG-HA-AGO2 (K525E)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    K525E

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FUW-TetO-Gata3

    Plasmid

    #128824

    Purpose

    dox-dependent expression of mouse Gata3

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-BEACON1

    Plasmid

    #171697

    Purpose

    Expresses BEACON1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W104A/Y132D

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.27_eSOX17

    Plasmid

    #195498

    Purpose

    Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Full distal endoderm enhancer region of SOX17 (SOX17 Human)
    

    Use

    Tags

    minP_luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.27_eSOX17.2

    Plasmid

    #195499

    Purpose

    Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Core distal endoderm enhancer region of SOX17 (SOX17 Human)
    

    Use

    Tags

    minP_luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX6P2-MDMX (1-120)

    Plasmid

    #62065

    Purpose

    Expression of human MDM2 (1-120) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 1-120

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only