We narrowed to 4,050 results for: bre

1. pBabe JUND-HA neo

   Plasmid

   #58489

   Purpose

   mammalian expression of human JUND

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   JUND (JUN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-rBrokenheart

   Plasmid

   #203394

   Purpose

   AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   Brokenheart
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-shLuc.mKO2

   Plasmid

   #85224

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-CMV-XRCC1-EGFP-Hygro

   Plasmid

   #176062

   Purpose

   EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   X-ray repair cross complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-OSF-PP-human-CHMP3

   Plasmid

   #154180

   Purpose

   expresses human CHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3 (CHMP3 Human)
   

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330 Rosa26 AscI LH291Gu1 (LH500)

   Plasmid

   #99628

   Purpose

   Plasmid used to target the pre-modified Rosa26 allele with Cas9

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMVA2

   Plasmid

   #119951

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A110G

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Puro-IKBalpha-wt

   Plasmid

   #15290

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-Rab8a-DN (T22N)

    Plasmid

    #101049

    Purpose

    Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    CMV

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Rab27B N133I

    Plasmid

    #89449

    Purpose

    Expression of GFP-tagged human Rab27B N133I in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B N133I (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    N133I

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe RFP1-KRT5 hygro

    Plasmid

    #58493

    Purpose

    mammalian expression of mRFP1-tagged human KRT5

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    KRT5 (KRT5 Human)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid

    #109428

    Purpose

    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Inserted 43 base pairs into mCherry to create rep…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid

    #133044

    Purpose

    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Use

    Tags

    NLS, TEV protease + TEV cleavage site, and miRFP6…

    Expression

    Mammalian

    Mutation

    S63K

    Promoter

    CAG

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rab27B T23N

    Plasmid

    #89450

    Purpose

    Expression of GFP-tagged human Rab27B T23N in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B T23N (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    T23N

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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