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FUGW-EGFP-mHif1a-T163A

Plasmid

#242810

Purpose

Lentiviral vector expressing mouse Hif1a-T163A mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T163A

Promoter

Ubiquitin C

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mHif1a-T163E

Plasmid

#242811

Purpose

Lentiviral vector expressing mouse Hif1a-T163E mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T163E

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET-p120RasGAP-714-1047-WT

Plasmid

#245307

Purpose

Expresses GAP domain of human p120RasGAP (residues 714-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-713

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLVXNeo_SPARC-mSc-SBP

Plasmid

#234885

Purpose

Expresses mScarlet-i and SBP tagged human SPARC for protein visualisation and use in the RUSH trafficking system

Depositor

Nicola Stevenson

Article

Thompson et al J Cell Biol. 2025 Oct 6;224(10):e2024111

Insert

SPARC (SPARC Human)

Use

Lentiviral

Tags

SBP and mScarleti

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG coca-5HT3-IRES-GFP

Plasmid

#242193

Purpose

Expression vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

coca-5HT3-IRES-GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

CAG

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Strep(II)(C)-CTR9

Plasmid

#231725

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CTR9 (CTR9 Human)

Tags

3C-Strep(II)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pOET3-6xHis-hSMSr

Plasmid

#236224

Purpose

Express N-terminal His-tagged human SMSr (SAMD8) protein in insect cells

Depositor

Chiaki Murakami

Article

Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

Insert

SAMD8 (SAMD8 Human)

Tags

Hexa histidine tag (6xHis-tag) and enterokinase s…

Expression

Insect

Mutation

wild type

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Nluc-_(PLRSKK)-CA1-19

Plasmid

#232657

Purpose

Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CGA (CGA Human)

Use

Luciferase

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Actin-C-18

Plasmid

#231772

Purpose

Visualization of actin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Actin-C-18 (ACTB Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Actin-C-18

Plasmid

#231780

Purpose

Visualization of actin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Actin-C-18 (ACTB Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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H6GB1tev-IntC-P53(304-393)

Plasmid

#200315

Purpose

bacterial expression of P53 TETCTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6GB1tev and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH

Plasmid

#148878

Purpose

Mammalian Expression of TNFalpha-ARE-polyA-Malat

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

TNFalpha-ARE-polyA-Malat (TNF Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100L-siResist

Plasmid

#49854

Purpose

Encodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100 mutated to Leucine. Contains severa…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
optoFcR

Plasmid

#224291

Purpose

Expression of an optogenetic Fc Receptor

Depositor

Meghan Morrissey

Article

Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

Insert

Fc receptor gamma (Fcer1g Mouse)

Use

Lentiviral

Tags

mScarlet-Cry2-olig and myristoylation sequence

Mutation

aa 45-86 only

Promoter

SFFV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Cyclin D3

Plasmid

#215116

Purpose

Expresses SS-Cyclin D3 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND3 (CCND3 Human)

Tags

SS (STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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NKP40 RFP (794)

Plasmid

#62006

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

C17orf32 (C17orf32 Human)

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
Human SERCA2b (pcDNA3.1+)

Plasmid

#75188

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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ptyrEGFP-TYR

Plasmid

#32782

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Tags

EGFP

Expression

Mammalian

Promoter

Tyrosinase

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
EGFR-GFP

Plasmid

#32751

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP

Depositor

Alexander Sorkin

Article

Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7

Insert

EGFR (EGFR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only

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