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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB302-er-mCherry

Plasmid

#186287

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa LCD

Plasmid

#197014

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (WT)

Plasmid

#197001

Purpose

Mammalian inducible expression construct of myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

-

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF1_F

Plasmid

#146281

Purpose

Bacterial Expression of DrUPF1

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF1 (upf1 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF3b_F

Plasmid

#146284

Purpose

Bacterial Expression of DrUPF3b

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF3b (upf3b Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA_D

Plasmid

#146140

Purpose

Mammalian Expression of tubcDNA

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC33_D

Plasmid

#146141

Purpose

Mammalian Expression of tubcDNA-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC33

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC62_D

Plasmid

#146142

Purpose

Mammalian Expression of tubcDNA-PTC62

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC62

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC62-deltaIntron3_D

Plasmid

#146146

Purpose

Mammalian Expression of tubgenomic-PTC62-delIntron3

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC62-delIntron3

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-767del664-673_J

Plasmid

#146671

Purpose

Bacterial Expression of HsPatL1_517-767-del664-673

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-767-del664-673 (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_70-770del399-516_J

Plasmid

#146682

Purpose

Mammalian Expression of HsPatL1_70-770del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1-del399-516_J

Plasmid

#146686

Purpose

Mammalian Expression of HsPatL1-del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-delta_E

Plasmid

#146181

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-PTC64-V5His6_E

Plasmid

#146183

Purpose

Insect Expression of DmAdh-PTC64

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh-PTC64 (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-V5His6_E

Plasmid

#146184

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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p32m3C Trx-CN

Plasmid

#196474

Purpose

Protein expression of Thiolredoxin-3C cleavage site-CarHCN

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

Trx-CN

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pCAG-mTurbo-mito

Plasmid

#187177

Purpose

Express mitochondira-localized miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

mtActA fused with miniTurbo (BirA mutant)

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mTurbo

Plasmid

#187178

Purpose

Express histone H2B tagged with miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

histone H2B fused with miniTurbo (BirA mutant)

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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IBAR-Cry2PHR

Plasmid

#176604

Purpose

Cry2PHR with N-terminal IBAR domain

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

IBAR-Cry2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN079

Plasmid

#167960

Purpose

express E. coli E69 (O9a:K30) Wzc with Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN072

Plasmid

#167953

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN073

Plasmid

#167954

Purpose

express E. coli E69 (O9a:K30) Wzc with Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y706E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN075

Plasmid

#167956

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN076

Plasmid

#167957

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN077

Plasmid

#167958

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN078

Plasmid

#167959

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-D-TOPO-kcnt2b

Plasmid

#164964

Purpose

Zebrafish kcnt2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt2b (LOC565691 Zebrafish)

Use

Pcr cloning vector

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C511-E15: attB5r-ffluc2-nostop-attB1r

Plasmid

#162939

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C413-E40: attB4-EFSp>-attB1r

Plasmid

#162928

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

short form of human EF1 alpha promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C413-E34: attB4-mCMVp>-attB1r

Plasmid

#162926

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

minimal CMV IE1 promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C238-E07: attB2r-SV40polyA-attB3

Plasmid

#162917

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 polyadenylation sequence

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C234-E24: attB2r-Strep2-attB3

Plasmid

#162913

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C231-E02: attB2r-NanoLuc-attB3

Plasmid

#162901

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C232-E06: attB2r-mCherry-attB3

Plasmid

#162903

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C453-E04: attB4-CMV51p>-attB5

Plasmid

#162973

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized human CMV promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C453-E17: attB4-PGKp>-attB5

Plasmid

#162976

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C413-E36: attB4-CMV51p>-attB1r

Plasmid

#162927

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized CMV promoter

Use

Synthetic Biology

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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