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pCMV-MtPylRS (human-opti)

Plasmid

#164080

Purpose

To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtPylRS-478(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MfBulKRS (human-opti)

Plasmid

#164196

Purpose

To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

Mf BulKRS(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Mutation

Y269A;Y347F

Promoter

CMV

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–301)

Plasmid

#186956

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–437)

Plasmid

#186958

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 L756A/I757A

Plasmid

#19938

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 L756A/I757A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutation of two adjacent hydrophobic residues, L…

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
alpha1Ddelta-G12-FRB

Plasmid

#87454

Purpose

rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

a1D short variants (Cacna1d Rat)

Tags

FRB

Expression

Mammalian

Mutation

delete KYPAKNTTIALQMLERML

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMiniT:myc-GluA1 TKIT donor

Plasmid

#169419

Purpose

Donor DNA for TKIT knockin of Myc to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _701-902

Plasmid

#86821

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMyc2ElbLuc,

Plasmid

#53244

Purpose

reporter plasmid containing 2 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc2ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Hyg-FHIT

Plasmid

#16410

Depositor

Bert Vogelstein

Article

Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

Insert

FHIT Hyg targeting (FHIT Human)

Use

AAV; Adeno-associated viral

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pBS human Frataxin (myc tag)

Plasmid

#11443

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Tags

myc

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBabe puro human Frataxin

Plasmid

#11505

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(-) + SLNCR1 _462-699

Plasmid

#86819

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _568-637

Plasmid

#86820

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRAN357

Plasmid

#120898

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-KD

Plasmid

#114064

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-KD (TTK Human)

Tags

His6

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1216 (USP)

Plasmid

#78588

Purpose

Expression flag-USP19 494-1216 (USP) in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1216 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1216 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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