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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Publication

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Publication

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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V517 pLP-dMyc SD - Acceptor

Plasmid

#11713

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V956 pLPS-3'ECFP - Acceptor

Plasmid

#11698

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP

Expression

Mammalian

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V795 pDNR MCS ES - Donor

Plasmid

#11696

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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Vector 1174D

Plasmid

#200012

Purpose

Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

Depositor

Omar Akbari

Publication

Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

Insert

Engineered dobulesex splicing module

Use

Synthetic Biology

Expression

Insect

Mutation

Engineered dobulesex splicing module

Promoter

Hr5IE1

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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V1579 pLP ProHis HTb - Acceptor

Plasmid

#11718

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

His

Expression

Bacterial

Available since

June 2, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V181 pLPS 3' Triple-Flag - Acceptor

Plasmid

#11708

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

Plasmid

#177841

Purpose

Mammalian Expression of Folate Receptor

Depositor

Karsten Melcher

Publication

Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

Insert

Folate receptor alpha (FOLR1 Human)

Expression

Mammalian

Mutation

all 11 C mutated to S

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + Needle

Plasmid

#84868

Purpose

Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Publication

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Needle

Use

Baculovirus

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA MOV10 K530A

Plasmid

#51716

Purpose

plasmid expresses FLAG/HA-tagged MOV10 K530A

Depositor

Markus Landthaler

Publication

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

K530A

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-PTD004

Plasmid

#34798

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

GTP-binding protein PTD004 (OLA1 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pADL*

Plasmid

#32097

Depositor

Christopher Buck

Publication

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Large T antigen

Expression

Mammalian

Mutation

57kT splice sites silently mutated, P156S to matc…

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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V308 pDNR MCS lacZ alpha - Donor

Plasmid

#11691

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA4/TO-cMYC

Plasmid

#216510

Purpose

Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Publication

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cMYC

Tags

HA

Expression

Mammalian

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pVRa34_1384

Plasmid

#49693

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF34_1384

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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ABE8.17-d

Plasmid

#136299

Purpose

expresses ABE8.17-d in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM2-C-19

Plasmid

#54163

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCM2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article