We narrowed to 15,050 results for: pET

1. pET28a-SznF H407A H409A H448A

   Plasmid

   #139566

   Purpose

   expressed SznF H407A H409A H448A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H407A H409A H448A
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b(+)_EGFP-LAMA-S98 (for p24)

   Plasmid

   #130717

   Purpose

   Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-S98
   

   Tags

   Hisx10 and StrepTag

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET151-GST-PP-RPN13 (19-132) K97E

   Plasmid

   #125825

   Purpose

   Expresses GST-RPN13 (19-132) K97E in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to glutamate

   Promoter

   T7

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET151-GST-PP-RPN13 (19-132) K83A

   Plasmid

   #125829

   Purpose

   Expresses GST-RPN13 (19-132) K83A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 83 to alanine

   Promoter

   T7

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET151-GST-PP-RPN13 (19-132) K97A

   Plasmid

   #125823

   Purpose

   Expresses GST-RPN13 (19-132) K97A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to alanine

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET151-GST-PP-RPN13 (19-132) K97D

   Plasmid

   #125824

   Purpose

   Expresses GST-RPN13 (19-132) K97D in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to aspartate

   Promoter

   T7

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET151-GST-PP-RPN13 (19-132) R104A

   Plasmid

   #125826

   Purpose

   Expresses GST-RPN13 (19-132) R104A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to alanine

   Promoter

   T7

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET151-GST-PP-RPN13 (19-132) R104D

   Plasmid

   #125827

   Purpose

   Expresses GST-RPN13 (19-132) R104D in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to aspartate

   Promoter

   T7

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET151-GST-PP-RPN13 (19-132) R104E

   Plasmid

   #125828

   Purpose

   Expresses GST-RPN13 (19-132) R104E in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to glutamate

   Promoter

   T7

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pet28a His6-HSPB7 (zebrafish) C49S

    Plasmid

    #110071

    Purpose

    Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 49 to Serine

    Promoter

    T7

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid

    #101240

    Purpose

    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FFSARG peptide, ddFLN4 fingerprint
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET11a asyn Q79N (TAC-->TAT)

    Plasmid

    #105733

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    Q79N

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet151D-TOPO-BROX 1-411; K141E

    Plasmid

    #89865

    Purpose

    Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated lysine 141 to glutamic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pet151D-TOPO-BROX 1-411; L212D

    Plasmid

    #89864

    Purpose

    Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated leucine 212 to aspartic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-GFP-PP-RPN2 (918-953)

    Plasmid

    #73743

    Purpose

    Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pET151-GFP-PP-RPN2 (932-953)

    Plasmid

    #73745

    Purpose

    Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pET151-GFP-PP-RPN2 (918-947)

    Plasmid

    #73747

    Purpose

    Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-947 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pET151-GFP-PP-RPN2 (797-953)

    Plasmid

    #74228

    Purpose

    [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pET151-Vps4(101-437) L151D

    Plasmid

    #87736

    Purpose

    Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Leu 151 to Asp

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET151-hUCH37 (ISF1)(M148D/F149D)

    Plasmid

    #71388

    Purpose

    Expression of hUCH37 (M148D/F149D) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
    

    Tags

    HIS-TEV sequence

    Expression

    Bacterial

    Mutation

    changed methionine 148 to aspartate, phenylalanin…

    Promoter

    T7

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pET151-hUCH37 (ISF1)(M148A/F149A)

    Plasmid

    #61933

    Purpose

    Expression of hUCH37 (M148A/F149A) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Methionine 148 and Phenylalanine 149 were mutated…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

    Plasmid

    #61941

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-117) (NFRKB Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 117, stop codon introdu…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

25. 213-petDuet1-GBD-LUMP--cdc42

    Plasmid

    #65973

    Purpose

    Dual expression of GBD-LUMP and cdc42

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Inserts

    LUMP
    cell division cycle 42 (CDC42 Human)
    

    Tags

    GBD (GTPase binding domain from ACK1; residues 44…

    Expression

    Bacterial

    Mutation

    constitutively active Cdc42 (Q61L)

    Promoter

    T7

    Available Since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-22HT-MSI1(7-192)Q30D

    Plasmid

    #60346

    Purpose

    Q30D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    Q30D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Dec. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pET-22HT-MSI1(7-192)R53E

    Plasmid

    #60352

    Purpose

    R53E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET-22HT-MSI1(7-192)G64A

    Plasmid

    #60351

    Purpose

    G64A mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G64A; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pET-22HT-MSI1(7-192)H83L

    Plasmid

    #60350

    Purpose

    H83L mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83L; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only