We narrowed to 8,429 results for: BLI;
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Plasmid#40421DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; F1908A, L1936R, I2131QPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
RTF1
Plasmid#36198PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRTF1 (RTF1 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains amino acid residues 347-482PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
PX398 Nitratifractor salsuginis str DSM 16511 Cas9
Plasmid#68334PurposeNitratifractor salsuginis str DSM 16511 Cas9DepositorInsertNsCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXC8 (murine) HIS-tag pET
Plasmid#8567DepositorInsertHOXC8 (Hoxc8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
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XE11 Xwnt 8b
Plasmid#16862DepositorInsertXwnt-8b (wnt8b Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
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PREX1
Plasmid#32041PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPREX1 (PREX1 )
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationfirst PDZ domain only (amino acids Residues 607-7…PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
rFIXX-bp chain A (IRESneo)
Plasmid#82450PurposeChain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.DepositorInsertrFIXX-bp chain A
UseTagsPolyhistidineExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
APPL1-N308D-mCherry
Plasmid#102784PurposePoint mutation in APPL1 that diminishes its interaction with Rab5DepositorInsertAPPL1 (APPL1 Human)
UseTagsmCherryExpressionMammalianMutationchanged asparagine 308 to aspartate; *(see below)PromoterCMVAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28-Nsp14-TEV-Strep
Plasmid#154953PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertNsp14-TEV-Strep (ORF1ab SARS-CoV-2)
UseTagsTEV-StrepExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22-Strep-TEV-Nsp10
Plasmid#154958PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertStrep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
UseTagsStrep-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
DYSF-Exon42-del
Plasmid#60194PurposePlasmid contains human dysferlin with exon 42 deleted. Deletion maintains the open reading frame.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationExon 42 has been deletedPromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
DYSF-Exon41-del
Plasmid#60193PurposePlasmid contains human dysferlin with exon 41 deleted. Deletion maintains the open reading frame.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationExon 41 has been deletedPromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Dox human Citron shRNA 1 mKate
Plasmid#155293PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652DepositorInsertCitron (CIT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse 5' Npm1 AID GFP PuroR
Plasmid#127899PurposeHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 GeneDepositorInsertNpm1 (Npm1 Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PIWIL2
Plasmid#32042PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPIWIL2 (PIWIL2 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPAZ domain only (amino acids 386-533)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pIC145
Plasmid#63068PurposeRetroviral expression of EGFP-TEV-S-tag CENP-O for protein localization and affinity purification (LAP)DepositorInsertCENP-O, IMAGE clone 3942844 (CENPO Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 KIBRA WW domain #2
Plasmid#104230PurposeBacterial expression of WW domain #2 from KIBRADepositorInsertKIBRA WW domain #2 (WWC1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain B (IRESpuro)
Plasmid#82451PurposeChain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.DepositorInsertrFIXX-bp chain B
UseTagsBiotinExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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HOXC5 (human) HIS-tag pET
Plasmid#8563DepositorInsertHOXC5 (HOXC5 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at Amino Acid #2 (Ser); makes protein by W…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUASP Flag Rab32 CA
Plasmid#44834DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsFlagExpressionInsectMutationQ79L (constitutive active)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
DYSF-Exon17-del
Plasmid#60182PurposePlasmid contains human dysferlin with exon 17 deleted. Deletion maintains the open reading frame.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationExon 17 has been deletedPromoterAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMB018 / LRRK2_BC_1309_2527_M2397T
Plasmid#40523DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTags6xHisExpressionMutationaa1309_2527; M2397TPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMB018 / LRRK2_BC_1185_2136
Plasmid#40539DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTags6xHisExpressionMutationWT; aa1185_2136PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCRII-Topo Cdh10 in situ probe
Plasmid#45638DepositorInsertCdh10 in situ probe (Cdh10 Mouse)
UseIn situTagsExpressionMutationfragment contains nt 2628-3061 of Cdh10 (GenBank …PromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103 YAP1 WW domain #2
Plasmid#104228PurposeBacterial expression of WW domain #2 from YAP1DepositorInsertYAP1 WW domain #2 (YAP1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 YAP1 WW domain #1
Plasmid#104227PurposeBacterial expression of WW domain #1 from YAP1DepositorInsertYAP1 WW domain #1 (YAP1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJK383
Plasmid#72474PurposeProduces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges tryptophan-17 to phenylalanine, tryptopha…PromoterrecAAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pattB-aubergine-ADH-gf
Plasmid#69448Purposegenomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead AubDepositorInsertaubergine (aub Fly)
UseTagsNAExpressionInsectMutationchr2L:10,998,626(−)T>GPromoteraubergineAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only