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We narrowed to 8,862 results for: BLI;

Showing: 6551 - 6600 of 8862 results

  1. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid
    #230055
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230055-plasmid-map-sequence-id-457173

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  2. pLPS-hSox2-gfp

    Plasmid
    #49390
    Purpose
    mammalian expression of human Sox2 fused to EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    49390-plasmid-map-sequence-id-354145

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  3. pAG426-GAL-Alpha-synuclein-E46K

    Plasmid
    #181723
    Purpose
    Galactose inducible expression of Alpha-synuclein-E46K
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    E46K
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    181723-plasmid-map-sequence-id-439543

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  4. DDX3X in pENTR

    Plasmid
    #216572
    Purpose
    gateway cloning donor vector containing DDX3X
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    DDX3X (DDX3X Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216572-plasmid-map-sequence-id-426667

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  5. Hs.KRAS4b G12C

    Plasmid
    #83130
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12C
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83130-plasmid-map-sequence-id-158093

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  6. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  8. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  9. huntingtin_FL_NbioCflag

    Plasmid
    #194767
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194767-plasmid-map-sequence-id-402615

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  10. Hs.NRAS G12V

    Plasmid
    #83174
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    neuroblastoma RAS viral oncogene homolog (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    83174-plasmid-map-sequence-id-158137

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  11. MS2_SC2_S_Delta

    Plasmid
    #175950
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Delta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175950-plasmid-map-sequence-id-345521

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  12. ALDH18A1

    Plasmid
    #38947
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH18A1 (ALDH18A1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. Hs.KRAS4b Q61H

    Plasmid
    #83140
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61H
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83140-plasmid-map-sequence-id-158103

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  14. CAMK2G

    Plasmid
    #38894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2G (CAMK2G Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pCDF1-mAtf4-HT-puro

    Plasmid
    #101795
    Purpose
    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4
    Depositor
    Seiichi Oyadomari
    Insert
    Activating transcription factor 4 (Atf4 Mouse)
    Use
    Lentiviral
    Tags
    Halo tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    101795-plasmid-map-sequence-id-194514

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  17. PE-SUMO CRYBB1

    Plasmid
    #217675
    Purpose
    For expression of SUMO tagged human beta B1 crystallin
    Depositor
    Larry David
    Insert
    CRYBB1 (CRYBB1 Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217675-plasmid-map-sequence-id-434867

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  18. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

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  20. PTPN11 in pENTR

    Plasmid
    #216604
    Purpose
    gateway cloning donor vector containing PTPN11
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    PTPN11 (PTPN11 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216604-plasmid-map-sequence-id-427162

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  21. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  22. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid
    #192838
    Purpose
    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192838-plasmid-map-sequence-id-378336

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  23. Hs.KRAS4b G12V

    Plasmid
    #83132
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83132-plasmid-map-sequence-id-158095

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  24. p5'Entry-lck-5.5kb

    Plasmid
    #58890
    Purpose
    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    Use
    Gateway 5' entry clone
    Tags
    Expression
    Mutation
    mutated single nucleotide (T-->C, 13th nucleot…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58890-plasmid-map-sequence-id-91281

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  25. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  26. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid
    #159912
    Purpose
    Mutagenesis of Kcnd3
    Depositor
    Larry Zweifel
    Insert
    Kcnd3 (Kcnd3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159912-plasmid-map-sequence-id-317319

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  27. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid
    #107107
    Purpose
    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.
    Depositor
    Michael E. Wright
    Insert
    human ubiquitin UBB (UBB Human)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Tet
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107107-plasmid-map-sequence-id-218027

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  29. psd44-G13QL

    Plasmid
    #46828
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q226L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46828-plasmid-map-sequence-id-137240

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  30. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  31. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid
    #181724
    Purpose
    Galactose inducible expression of Alpha-synuclein-H50Q
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H50Q
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181724-plasmid-map-sequence-id-431239

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  33. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

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  34. CD44Z-c011

    Plasmid
    #175455
    Purpose
    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175455-plasmid-map-sequence-id-353216

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  35. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Use
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  36. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has Service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105715-plasmid-map-sequence-id-207274

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  37. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  38. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110420-plasmid-map-sequence-id-213695

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  40. pBabe-Kras G12V-T35S

    Plasmid
    #58900
    Purpose
    Expresses human Kras with G12V and T35S mutations
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G12V and T35S mutations (effector binding mutant)
    Promoter
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  41. Hs.KRAS4b S17N

    Plasmid
    #83156
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S17N
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83156-plasmid-map-sequence-id-158119

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  42. Hs.KRAS4b G12R

    Plasmid
    #83143
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12R
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83143-plasmid-map-sequence-id-158106

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  44. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  45. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  46. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pAG426-GAL-Alpha-synuclein-A53T

    Plasmid
    #181722
    Purpose
    Galactose inducible expression of Alpha-synuclein-A53T
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    A53T
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    181722-plasmid-map-sequence-id-439443

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  49. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  50. Hs.KRAS4b Q61L

    Plasmid
    #83134
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61L
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83134-plasmid-map-sequence-id-158097

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Showing: 6551 - 6600 of 8862 results