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pXTK015 [Marker_SacB_PartType5-7]

Plasmid

#229252

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SacB_PartType5-7

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

SacB

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK016 [Marker_SacB_PartType8-10]

Plasmid

#229253

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SacB_PartType8-10

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

SacB

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#227773

Purpose

Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and tp1

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAM201

Plasmid

#227660

Purpose

pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3x-FLAG-HRV3C-

Expression

Yeast

Mutation

(amino acids 121-131) VTENKIF mutated to AAAAAAA

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBsVchDonor

Plasmid

#203814

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

Mini-Tn, aprE cassette

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM315 pACYC-His6-Rpn10[ΔUIM]

Plasmid

#226348

Purpose

Expression of yeast Rpn10 with the UIM deleted

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

rpn10 (RPN10 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

delta UIM

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAM227

Plasmid

#226650

Purpose

MSS-His6-UFD1L

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

UFD1 (UFD1 Human)

Tags

His-thrombin

Expression

Bacterial

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAG66

Plasmid

#226751

Purpose

Expresses exogenous GFP

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

GFP

Use

Lentiviral

Tags

sfGFP

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_dRibose

Plasmid

#215561

Purpose

Expresses GFP in response to D-Ribose

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
LcLacI

Expression

Bacterial

Promoter

P250 and Plclaci

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYGS Q16C

Plasmid

#217674

Purpose

For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

Depositor

Larry David

Insert

CRYGS (CRYGS Human)

Tags

6XHis and SUMO

Expression

Bacterial

Mutation

Glutamine 16 changed to Cysteine

Promoter

T7

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS8

Plasmid

#218716

Purpose

Expresses N-terminally EGFP-tagged BBS8 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS8 (TTC8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGM209

Plasmid

#220174

Purpose

Expresses exogenous wild-type TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PM-Ras-LOCKR-PL Key

Plasmid

#216504

Purpose

Ras activity dependent proximity labeler, Key only, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-PL Key

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-PL Key

Plasmid

#216505

Purpose

Ras activity dependent proximity labeler, Key only, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-PL Key

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ER-Ras-LOCKR-PL Key

Plasmid

#216506

Purpose

Ras activity dependent proximity labeler, Key only, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-PL Key

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito-Ras-LOCKR-PL Key

Plasmid

#216507

Purpose

Ras activity dependent proximity labeler, Key only, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-PL

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDY380

Plasmid

#182962

Purpose

Amp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

ptsI

Use

Synthetic Biology

Tags

GFP

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDY390

Plasmid

#182963

Purpose

Amp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

ptsI

Use

Synthetic Biology

Tags

GFP

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 AvrRpm1

Plasmid

#209185

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AvrRpm1

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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