We narrowed to 13,780 results for: TIM

1. pLV_ADRB2-mTurquoise2-iLID

   Plasmid

   #161002

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   ADRB2-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LEPZ

   Plasmid

   #111161

   Purpose

   miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ShuttleNSE

   Plasmid

   #50958

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   NSE

   Available since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pZR069_CROPseq-sgPTPRC-CRISPRi

   Plasmid

   #180271

   Purpose

   Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   PTPRC (CD45) sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript-mouse cyclin D2

   Plasmid

   #86791

   Purpose

   bacterial expression of mouse cyclin D2

   Depositor

   Martine Roussel, Charles Sherr

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D2 (Ccnd2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163043

   Purpose

   QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSTAR-mOrange2-sfGFP

   Plasmid

   #115693

   Purpose

   Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

   Depositor

   David Suter

   Article

   Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

   Insert

   SA-mOrange2-sfGFP-SD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ER-mCherry-eMagA

   Plasmid

   #162248

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   CytP450 ER targeting sequencel-mCherry-eMagA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBRBB-eGFP

    Plasmid

    #32549

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT3REVIR

    Plasmid

    #111176

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eMagA-mCherry-Mito

    Plasmid

    #162251

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagA-mCherry-OMP25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPaxillin-Electra1-N1

    Plasmid

    #179484

    Purpose

    Expresses paxillin fused to blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    paxillin-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-ChR2(H134R)-GFP

    Plasmid

    #58880

    Purpose

    AAV expression of ChR2(H134R)-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Has Service

    AAV Retrograde and AAV8

    Insert

    ChR2(H134R)-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-CoChR-mRuby2-ST

    Plasmid

    #109131

    Purpose

    Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CoChR-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNubWtXgate

    Plasmid

    #105079

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PKG I alpha WT

    Plasmid

    #16390

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_112

    Plasmid

    #136475

    Purpose

    Lentiviral expression of AsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge