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FUW-TetO-Klf5

Plasmid

#128830

Purpose

dox-dependent expression of mouse Klf5

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Klf5 (Klf5 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-Hygro

Plasmid

#176056

Purpose

EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

Plasmid

#198035

Purpose

Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

SINTBAD (TBKBP1 Human)

Tags

EGFP and GST-TEV

Expression

Insect

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Foff hChR2(H134R)-EYFP

Plasmid

#55647

Purpose

Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

Short Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-HA

Plasmid

#242217

Purpose

Expression of full-length human β-catenin using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-iC++-EYFP

Plasmid

#137155

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon/Von eYFP

Plasmid

#137164

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV Retrograde and AAV8

Insert

3x-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183521

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

H33R

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC(WT)

Plasmid

#223742

Purpose

Expression of recombinant protein for purification, codon optimized for Insect cells

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Src Kinase (SRC Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#74245

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

Plasmid

#223211

Purpose

Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

CD25 (IL2RA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TRPML1(PHD)

Plasmid

#221752

Purpose

Stable expression of GFP-TRPML1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

TRPML1 (MCOLN1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TRPML1(PHD) (amino acids 1–69)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-BFP

Plasmid

#137131

Purpose

Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_6xHis-TEV-mCh-NDP52

Plasmid

#187829

Purpose

Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHisTag, TEV, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-Mito-7

Plasmid

#56231

Purpose

Localization: Mitochondria, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Mito (COX8A Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183519

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Has Service

AAV8

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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