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Plasmid#188304PurposeExpression of Luz via a T7 promoter in TXTLDepositorInsertluz
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.601
Plasmid#184955PurposeExpress -Eco1 ncRNA library variantsDepositorInsertEco1 ncRNA (variants)
UseTagsExpressionBacterialMutationLibrary members with mutations described in manus…PromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
hPCNA-S228I
Plasmid#190938Purposeuntagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsExpressionBacterialMutationchanged serine 228 to isoleucinePromoterT7Available sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuzGFPHis
Plasmid#188315PurposeExpression of Luz-eGFP via a T7 promoter in TXTLDepositorInsertLuz-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HisGFPLuz
Plasmid#188314PurposeExpression of eGFP-Luz via a T7 promoter in TXTLDepositorInsertGFP-Luz fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2116
Plasmid#170393PurposePlasmid for genetic complementation of LetAB with ΔRing 1 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ48-159)PromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-pvdF WT
Plasmid#187974PurposeExpresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosaDepositorInsertpvdF WT
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CylK-Strep
Plasmid#185962PurposeExpresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparationDepositorInsertCylK
UseTagsHis6-SumoExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK C-term
Plasmid#186147PurposeFor expresseion of recombinant FAK c-terminal domain in E. coliDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S19-T48C
Plasmid#128597PurposeExpresses tagless S19-T48C mutant 30S ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S19 (rpsS E.coli)
UseTagsExpressionBacterialMutationT48CPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S15-T79C
Plasmid#133048PurposeExpresses tagless S15-T79C mutant ribosomal protein in E.coliDepositorInsertS15 E.coli ribosomal protein (rpsO E.coli)
UseTagsExpressionBacterialMutationT79CPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S3-M129C
Plasmid#132949PurposeExpresses tagless S3-M129C mutant ribosomal protein in E.coliDepositorInsertE.coli ribosomal protein S3 (rpsC E.coli)
UseTagsExpressionBacterialMutationM129CPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CcCDP_p15A
Plasmid#179272PurposeExpresses CcCDP in E. coli BL21(DE3)DepositorInsertcellodextrin phosphorylase
UseSynthetic BiologyTags6xHISExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014
Plasmid#105637PurposeExpresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmidDepositorUseTagsExpressionBacterialMutationMutations: F11S, G12A, V132K, C133V, A135KPromoterT7Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-epELM-5
Plasmid#179391PurposeExpress a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteriaDepositorInsertELMSAN1 (MIDEAS Human)
UseTags6x HisExpressionBacterialMutationPromoterT7 lacAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term SMT3 NikJC199U
Plasmid#174210PurposeExpression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term SMT3 NikJC199U
Plasmid#174362PurposeExpression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP NikJC199U
Plasmid#174364PurposeExpression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
NmMetNI-WT
Plasmid#172114PurposeExpression of MetN and MetI membrane proteinsDepositorInsertsMetN
MetI
UseTags10x His Tag and EnterokinaseExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF1 (G180A)
Plasmid#175415PurposeEncodes His-MBP-TEV-ybbR-G180A eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutationG180APromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF1 (WT)
Plasmid#175414PurposeEncodes His-MBP-TEV-ybbR-WT eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-yDrp1-StrepII
Plasmid#174427PurposeExpresses S. pombe Drp1 in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj20
Plasmid#173150PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj20 (si:ch211-113j13.2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12b
Plasmid#173142PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12b (kcnj12b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj14
Plasmid#173144PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj14 (kcnj14 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj19a
Plasmid#173148PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19a (kcnj19a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj19b
Plasmid#173149PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19b (kcnj19b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj21
Plasmid#173151PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj21 (zgc:162160 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 endAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj2a
Plasmid#173127PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj2a (kcnj2a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj2b
Plasmid#173128PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj2b (kcnj2b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj3b
Plasmid#173130PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj3b (kcnj3b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj4
Plasmid#173131PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj4 (kcnj4 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj9
Plasmid#173135PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj9 (kcnj9 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10a
Plasmid#173137PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10a (kcnj10a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10b
Plasmid#173138PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10b (LOC100329607 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11l
Plasmid#173140PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11l (kcnj11l Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
Plasmid#165029PurposeExpress cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only