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pD649-HAsp-PSG11-Fc(DAPA)-AviTag-6xHis

Plasmid

#156740

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG11 (PSG11 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH2NLA-Fc(DAPA)-AviTag-6xHis

Plasmid

#156634

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH2NLA (NOTCH2NLA Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SDK2(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157613

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SDK2 (SDK2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ZPBP-Fc(DAPA)-AviTag-6xHis

Plasmid

#156747

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ZPBP (ZPBP Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SDK1(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157615

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SDK1 (SDK1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PAPLN(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157603

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PAPLN (PAPLN Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis

Plasmid

#157388

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG9 (PSG9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM8-COMP5AP-AviTag-9xHis

Plasmid

#157294

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM8 (CEACAM8 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NFAM1-COMP5AP-AviTag-9xHis

Plasmid

#157066

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NFAM1 (NFAM1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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CRYZL1_pLENTI-CAG-IRES-GFP

Plasmid

#177004

Purpose

Mammalian lentiviral expression vector encoding CRYZL1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CRYZL1 (CRYZL1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CRYZL1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176941

Purpose

Mammalian expression vector encoding CRYZL1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CRYZL1 (CRYZL1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-OLIG2-Adel-IDR

Plasmid

#145249

Purpose

Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

OLIG2-Adel-IDR (OLIG2 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgMARK4-A

Plasmid

#138676

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgMARK4-B

Plasmid

#138677

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgMARK4

Plasmid

#138707

Purpose

Expresses a human MARK4-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 human (MARK4 Human)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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MARK4 gRNA (BRDN0001148083)

Plasmid

#76729

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK4 (MARK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GK5 gRNA (BRDN0001146859)

Plasmid

#75773

Purpose

3rd generation lentiviral gRNA plasmid targeting human GK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GK5 (GK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GK5 gRNA (BRDN0001148146)

Plasmid

#75774

Purpose

3rd generation lentiviral gRNA plasmid targeting human GK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GK5 (GK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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