We narrowed to 10,458 results for: T7

1. pHN781

   Plasmid

   #178828

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A (AR Human)
   

   Use

   Tags

   His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sumire-pRsetB

   Plasmid

   #193361

   Purpose

   GFP variant emit 414 nm fluorescence.

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   Sumire
   

   Use

   E.coli expression

   Tags

   His X6 tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-nanoHER2-SpyTag003

   Plasmid

   #216312

   Purpose

   Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   nanoHER2-SpyTag003
   

   Use

   Tags

   His6 tag and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SIRT5-pET15b

   Plasmid

   #81030

   Purpose

   Bacterial expression of Human WT SIRT5 protein

   Depositor

   David Lombard

   Article

   Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

   Insert

   Sirt5 (SIRT5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Non tagged Avidin

   Plasmid

   #214835

   Purpose

   This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Non tagged Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACM4

   Plasmid

   #49797

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Use

   Tags

   S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-sfGFP

   Plasmid

   #199171

   Purpose

   used to express a superfolder GFP in E. coli for comparison to supercharged variants

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   superfolder GFP
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 19 pCOLI_A_SUMO

    Plasmid

    #160257

    Purpose

    N-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 18 pCOLI_A_Trx

    Plasmid

    #160255

    Purpose

    N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 23 pCOLI_A_N-SUMO_C_STREP

    Plasmid

    #160259

    Purpose

    N-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII, 6xHis, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3CEG

    Plasmid

    #60107

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BIRC6:SDC136-F10:C41228 (BIRC6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pRSETA_ionRD

    Plasmid

    #172924

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJSRSF3

    Plasmid

    #64692

    Purpose

    His-GST-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, GST, HisTag, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHMGWA-Pa14Csy4H29A

    Plasmid

    #41092

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Use

    Tags

    His6-MBP

    Expression

    Mutation

    His29Ala mmutation to abolish ribonuclease activi…

    Promoter

    T7

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET28c-F30-2xdBroccoli

    Plasmid

    #66843

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHMM

    Plasmid

    #67717

    Purpose

    Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Hexahistidine/Maltose binding protein/MS2 coat protein fusion
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-thr-RAF1-RBD

    Plasmid

    #86033

    Purpose

    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

    Depositor

    Dominic Esposito

    Insert

    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains residues 1-149

    Promoter

    T7

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. bfloGFPa1

    Plasmid

    #60749

    Purpose

    Bacterial expression of Amphioxus GFPa1

    Depositor

    Dimitri Deheyn

    Article

    Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

    Insert

    GFPa1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only