We narrowed to 9,396 results for: pho;

1. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Fes-SH2

   Plasmid

   #46426

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Fes (FES Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 396-497

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV HA hRB delta CDK + S780

   Plasmid

   #58915

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S780 restored

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3_shRNA resistant PKA-Cα-mEGFP

   Plasmid

   #114143

   Purpose

   Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
   

   Expression

   Mammalian

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX p85a(N)-SH2

   Plasmid

   #46464

   Depositor

   Leszek Kotula, Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   p85a(N) (PIK3R1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 321-434

   Promoter

   Tac

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pClneoMyc MOB1 T12/35A

   Plasmid

   #47938

   Purpose

   expresses Myc tagged human MOB1 containing T12A and T35A mutations

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T12A and T35A

   Promoter

   CMV

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

7. GFP-Braf R188L

   Plasmid

   #118330

   Purpose

   Expression of human Braf

   Depositor

   Philip Stork

   Article

   Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

   Insert

   Braf R188L (BRAF Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R188L

   Promoter

   CMV

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T Doc2beta C2AB(125) G361C

   Plasmid

   #134694

   Purpose

   Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Double C2 protein beta (Doc2b Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G361C, C145A, C217A, C249A, C290A, C338A, C387A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Cx43-Myc

   Plasmid

   #60693

   Purpose

   mammalian expression of Myc-tagged rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGEX SHP-1(N)-SH2

    Plasmid

    #46495

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(N) (PTPN6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-109

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DF-UAS-sspa-GCaMP6f

    Plasmid

    #67569

    Purpose

    Zebrafish expression of a short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-C3-PLK4-S305E-3xFLAG

    Plasmid

    #69840

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S305E

    Promoter

    CMV

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV HA hRB delta CDK + S811

    Plasmid

    #58919

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S811 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid

    #69841

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S285A/T289A

    Promoter

    CMV

    Available Since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GB1-PRD(703-868, P801G)-Strep

    Plasmid

    #164444

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV HA hRB delta CDK + S807

    Plasmid

    #58918

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S807 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCMV HA hRB delta CDK + S788

    Plasmid

    #58916

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S788 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV HA hRB delta CDK + T356

    Plasmid

    #58911

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T356 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only