We narrowed to 17,384 results for: puromycin

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pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196079

Purpose

Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196085

Purpose

Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P20

Plasmid

#120031

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

Plasmid

#70659

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-puro-crRNA

Plasmid

#194965

Purpose

The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_Puro_Telo_v1

Plasmid

#195138

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX305-pGK-N-dTAG-HA-PSMF1(1-271)_PuroR

Plasmid

#251194

Purpose

Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 and selection with Puromycin

Depositor

Wade Harper

Article

Adolf et al bioRxiv 2026.05.28.728263

Insert

PSMF1 (PSMF1 Human)

Use

Lentiviral

Tags

dTAG(FKBP12F36V)-HA

Expression

Mammalian

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH1

Plasmid

#241994

Purpose

Sleeping Beauty vector for inducible expression of a chicken H1 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H1

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH3

Plasmid

#241995

Purpose

Sleeping Beauty vector for inducible expression of a chicken H3 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H3

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH2B

Plasmid

#241996

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2B

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH2A

Plasmid

#241997

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2A targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2A

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-1-shH2B

Plasmid

#240417

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2B

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH4

Plasmid

#240431

Purpose

Sleeping Beauty vector for inducible expression of a chicken H4 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H4

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shCtrl

Plasmid

#240580

Purpose

Sleeping Beauty vector for inducible expression of a unspecific control shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

Scramble shRNA

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV152

Plasmid

#179916

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV51

Plasmid

#179915

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165888

Purpose

Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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